Differential effects of inulin or its fermentation metabolites on gut barrier and immune function of porcine intestinal epithelial cells

Prebiotics can modulate gut fermentation and improve intestinal barrier function in mammals. First, inulin fermentation profile was tested in a three-step in vitro model of the piglet’s gastro-intestinal tract combining a hydrolysis – dialysis step to a batch fermentation. Then, the differential eff...

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Main Authors: Julie Uerlings, Martine Schroyen, Els Willems, Sofie Tanghe, Geert Bruggeman, Jérôme Bindelle, Nadia Everaert
Format: Article
Language:English
Published: Elsevier 2020-04-01
Series:Journal of Functional Foods
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1756464620300797
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author Julie Uerlings
Martine Schroyen
Els Willems
Sofie Tanghe
Geert Bruggeman
Jérôme Bindelle
Nadia Everaert
author_facet Julie Uerlings
Martine Schroyen
Els Willems
Sofie Tanghe
Geert Bruggeman
Jérôme Bindelle
Nadia Everaert
author_sort Julie Uerlings
collection DOAJ
description Prebiotics can modulate gut fermentation and improve intestinal barrier function in mammals. First, inulin fermentation profile was tested in a three-step in vitro model of the piglet’s gastro-intestinal tract combining a hydrolysis – dialysis step to a batch fermentation. Then, the differential effects of digested inulin (after the hydrolysis – dialysis steps) or fermented inulin (after the fermentation step) on the expression of gut barrier and immune-related genes of IPEC-J2 cells were investigated by high-throughput qPCR. Inulin was associated with elevated short-chain fatty acids and butyrate levels. Upregulated expressions of tight and adherens junction genes were observed in IPEC-J2 cells supplemented with inulin fermentation supernatant compared to control IPEC-J2 cells and digested inulin. Therefore, metabolites arising from the fermentation process, including butyrate, could be responsible for the reinforcement of the barrier function.
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spelling doaj.art-a8a4eaf6fe5245c992cd757cbbb2d12f2022-12-21T22:32:27ZengElsevierJournal of Functional Foods1756-46462020-04-0167103855Differential effects of inulin or its fermentation metabolites on gut barrier and immune function of porcine intestinal epithelial cellsJulie Uerlings0Martine Schroyen1Els Willems2Sofie Tanghe3Geert Bruggeman4Jérôme Bindelle5Nadia Everaert6Precision Livestock and Nutrition Unit, TERRA Teaching and Research Centre, Gembloux Agro-Bio Tech, ULiège, Gembloux, Belgium; Research Foundation for Industry and Agriculture, National Scientific Research Foundation (FRIA-FNRS), Brussels, BelgiumPrecision Livestock and Nutrition Unit, TERRA Teaching and Research Centre, Gembloux Agro-Bio Tech, ULiège, Gembloux, BelgiumRoyal Agrifirm Group, Apeldoorn, the NetherlandsRoyal Agrifirm Group, Apeldoorn, the NetherlandsRoyal Agrifirm Group, Apeldoorn, the NetherlandsPrecision Livestock and Nutrition Unit, TERRA Teaching and Research Centre, Gembloux Agro-Bio Tech, ULiège, Gembloux, BelgiumPrecision Livestock and Nutrition Unit, TERRA Teaching and Research Centre, Gembloux Agro-Bio Tech, ULiège, Gembloux, Belgium; Corresponding author at: Gembloux Agro-Bio Tech, Passage des Déportés, 2, 5030, Gembloux, Belgium.Prebiotics can modulate gut fermentation and improve intestinal barrier function in mammals. First, inulin fermentation profile was tested in a three-step in vitro model of the piglet’s gastro-intestinal tract combining a hydrolysis – dialysis step to a batch fermentation. Then, the differential effects of digested inulin (after the hydrolysis – dialysis steps) or fermented inulin (after the fermentation step) on the expression of gut barrier and immune-related genes of IPEC-J2 cells were investigated by high-throughput qPCR. Inulin was associated with elevated short-chain fatty acids and butyrate levels. Upregulated expressions of tight and adherens junction genes were observed in IPEC-J2 cells supplemented with inulin fermentation supernatant compared to control IPEC-J2 cells and digested inulin. Therefore, metabolites arising from the fermentation process, including butyrate, could be responsible for the reinforcement of the barrier function.http://www.sciencedirect.com/science/article/pii/S1756464620300797PrebioticIn vitro fermentationPorcine intestinal epithelial cellsShort-chain fatty acidsGut barrierInflammatory response
spellingShingle Julie Uerlings
Martine Schroyen
Els Willems
Sofie Tanghe
Geert Bruggeman
Jérôme Bindelle
Nadia Everaert
Differential effects of inulin or its fermentation metabolites on gut barrier and immune function of porcine intestinal epithelial cells
Journal of Functional Foods
Prebiotic
In vitro fermentation
Porcine intestinal epithelial cells
Short-chain fatty acids
Gut barrier
Inflammatory response
title Differential effects of inulin or its fermentation metabolites on gut barrier and immune function of porcine intestinal epithelial cells
title_full Differential effects of inulin or its fermentation metabolites on gut barrier and immune function of porcine intestinal epithelial cells
title_fullStr Differential effects of inulin or its fermentation metabolites on gut barrier and immune function of porcine intestinal epithelial cells
title_full_unstemmed Differential effects of inulin or its fermentation metabolites on gut barrier and immune function of porcine intestinal epithelial cells
title_short Differential effects of inulin or its fermentation metabolites on gut barrier and immune function of porcine intestinal epithelial cells
title_sort differential effects of inulin or its fermentation metabolites on gut barrier and immune function of porcine intestinal epithelial cells
topic Prebiotic
In vitro fermentation
Porcine intestinal epithelial cells
Short-chain fatty acids
Gut barrier
Inflammatory response
url http://www.sciencedirect.com/science/article/pii/S1756464620300797
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