Development and application of a low-priced duplex quantitative PCR assay based on SYBR Green I for the simultaneous detection of porcine deltacoronavirus and porcine sapelovirus

Development and application of a low-priced duplex quantitative PCR assay based on SYBR Green I for the simultaneous detection of porcine deltacoronavirus and porcine sapelovirus

Porcine deltacoronavirus (PDCoV) and porcine sapelovirus (PSV) are two viruses that can cause diarrhoea in pigs and bring great economic loss to the pig industry. In this research, a duplex real-time quantitative polymerase chain reaction (qPCR) assay based on SYBR Green І was developed to simultane...

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Bibliographic Details
Main Authors: SJ Lu, MY Ma, XG Yan, FJ Zhao, WY Hu, QW Ding, HJ Ren, YQ Xiang, LL Zheng
Format: Article
Language:English
Published: Czech Academy of Agricultural Sciences 2023-03-01
Series:Veterinární Medicína
Subjects:
duplex real-time qpcr
pdcov
psv
Online Access:https://vetmed.agriculturejournals.cz/artkey/vet-202303-0002_development-and-application-of-a-low-priced-duplex-quantitative-pcr-assay-based-on-sybr-green-i-for-the-simulta.php

Internet

https://vetmed.agriculturejournals.cz/artkey/vet-202303-0002_development-and-application-of-a-low-priced-duplex-quantitative-pcr-assay-based-on-sybr-green-i-for-the-simulta.php

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