Transient disome complex formation in native polysomes during ongoing protein synthesis captured by cryo-EM

Transient disome complex formation in native polysomes during ongoing protein synthesis captured by cryo-EM

Abstract Structural studies of translating ribosomes traditionally rely on in vitro assembly and stalling of ribosomes in defined states. To comprehensively visualize bacterial translation, we reactivated ex vivo-derived E. coli polysomes in the PURE in vitro translation system and analyzed the acti...

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Main Authors: Timo Flügel, Magdalena Schacherl, Anett Unbehaun, Birgit Schroeer, Marylena Dabrowski, Jörg Bürger, Thorsten Mielke, Thiemo Sprink, Christoph A. Diebolder, Yollete V. Guillén Schlippe, Christian M. T. Spahn
Format: Article
Language:English
Published: Nature Portfolio 2024-02-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-024-46092-3

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https://doi.org/10.1038/s41467-024-46092-3

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