PbPH5, an H+ P-ATPase on the tonoplast, is related to malic acid accumulation in pear fruit
Organic acid content is one of the most important factors influencing fruit flavors. The predominant organic acid in most pear cultivars is malic acid, but the mechanism controlling its accumulation remains unclear. In this study, by comparing gene expression levels and organic acid contents, we fou...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311921637905 |
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| author | Jun-xing SONG Ying-can CHEN Zhao-hui LU Guang-ping ZHAO Xiao-li WANG Rui ZHAI Zhi-gang WANG Cheng-quan YANG Ling-fei XU |
| author_facet | Jun-xing SONG Ying-can CHEN Zhao-hui LU Guang-ping ZHAO Xiao-li WANG Rui ZHAI Zhi-gang WANG Cheng-quan YANG Ling-fei XU |
| author_sort | Jun-xing SONG |
| collection | DOAJ |
| description | Organic acid content is one of the most important factors influencing fruit flavors. The predominant organic acid in most pear cultivars is malic acid, but the mechanism controlling its accumulation remains unclear. In this study, by comparing gene expression levels and organic acid contents, we found that the expression of PbPH5, which encodes a P3A-ATPase, is highly correlated with malic acid accumulation in four different pear species, with correlation coefficients of 0.932**, 0.656*, 0.900**, and 0.518* (*, P<0.05; **, P<0.01) for Pyrus bretschneideri Rehd., P. communis Linn., P. pyrifolia Nakai., and P. ussuriensis Maxim., respectively. Moreover, the overexpression of PbPH5 in pear significantly increased the malic acid content. In contrast, silencing PbPH5 via RNA interference significantly decreased both its transcript level and the pear fruit malic acid content. A subcellular localization analysis indicated that PbPH5 is located in the tonoplast. Additionally, a phylogenetic analysis indicated that PbPH5 is a PH5 homolog gene that is clustered with the Petunia hybrida, Malus domestica, and Citrus reticulata genes. Considered together, these findings suggest that PbPH5 is a functionally conserved gene. Furthermore, the accumulation of malic acid in pear fruit is at least partly related to changes in the PbPH5 transcription levels. |
| first_indexed | 2024-12-12T20:57:06Z |
| format | Article |
| id | doaj.art-a8d306e6df304dafb8841a06a463b625 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-12T20:57:06Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-a8d306e6df304dafb8841a06a463b6252022-12-22T00:12:16ZengElsevierJournal of Integrative Agriculture2095-31192022-06-0121616451657PbPH5, an H+ P-ATPase on the tonoplast, is related to malic acid accumulation in pear fruitJun-xing SONG0Ying-can CHEN1Zhao-hui LU2Guang-ping ZHAO3Xiao-li WANG4Rui ZHAI5Zhi-gang WANG6Cheng-quan YANG7Ling-fei XU8College of Horticulture, Northwest A&F University, Yangling 712100, P.R.ChinaCollege of Horticulture, Northwest A&F University, Yangling 712100, P.R.ChinaCollege of Horticulture, Northwest A&F University, Yangling 712100, P.R.ChinaCollege of Horticulture, Northwest A&F University, Yangling 712100, P.R.ChinaCollege of Horticulture, Northwest A&F University, Yangling 712100, P.R.ChinaCollege of Horticulture, Northwest A&F University, Yangling 712100, P.R.ChinaCollege of Horticulture, Northwest A&F University, Yangling 712100, P.R.ChinaCorrespondence YANG Cheng-quan, Tel: +86-29-87081023; College of Horticulture, Northwest A&F University, Yangling 712100, P.R.ChinaCorrespondence XU Ling-fei, Tel: +86-29-87081023; College of Horticulture, Northwest A&F University, Yangling 712100, P.R.ChinaOrganic acid content is one of the most important factors influencing fruit flavors. The predominant organic acid in most pear cultivars is malic acid, but the mechanism controlling its accumulation remains unclear. In this study, by comparing gene expression levels and organic acid contents, we found that the expression of PbPH5, which encodes a P3A-ATPase, is highly correlated with malic acid accumulation in four different pear species, with correlation coefficients of 0.932**, 0.656*, 0.900**, and 0.518* (*, P<0.05; **, P<0.01) for Pyrus bretschneideri Rehd., P. communis Linn., P. pyrifolia Nakai., and P. ussuriensis Maxim., respectively. Moreover, the overexpression of PbPH5 in pear significantly increased the malic acid content. In contrast, silencing PbPH5 via RNA interference significantly decreased both its transcript level and the pear fruit malic acid content. A subcellular localization analysis indicated that PbPH5 is located in the tonoplast. Additionally, a phylogenetic analysis indicated that PbPH5 is a PH5 homolog gene that is clustered with the Petunia hybrida, Malus domestica, and Citrus reticulata genes. Considered together, these findings suggest that PbPH5 is a functionally conserved gene. Furthermore, the accumulation of malic acid in pear fruit is at least partly related to changes in the PbPH5 transcription levels.http://www.sciencedirect.com/science/article/pii/S2095311921637905pearP3A-ATPasePH5 homologmalic acid accumulationproton pump |
| spellingShingle | Jun-xing SONG Ying-can CHEN Zhao-hui LU Guang-ping ZHAO Xiao-li WANG Rui ZHAI Zhi-gang WANG Cheng-quan YANG Ling-fei XU PbPH5, an H+ P-ATPase on the tonoplast, is related to malic acid accumulation in pear fruit Journal of Integrative Agriculture pear P3A-ATPase PH5 homolog malic acid accumulation proton pump |
| title | PbPH5, an H+ P-ATPase on the tonoplast, is related to malic acid accumulation in pear fruit |
| title_full | PbPH5, an H+ P-ATPase on the tonoplast, is related to malic acid accumulation in pear fruit |
| title_fullStr | PbPH5, an H+ P-ATPase on the tonoplast, is related to malic acid accumulation in pear fruit |
| title_full_unstemmed | PbPH5, an H+ P-ATPase on the tonoplast, is related to malic acid accumulation in pear fruit |
| title_short | PbPH5, an H+ P-ATPase on the tonoplast, is related to malic acid accumulation in pear fruit |
| title_sort | pbph5 an h p atpase on the tonoplast is related to malic acid accumulation in pear fruit |
| topic | pear P3A-ATPase PH5 homolog malic acid accumulation proton pump |
| url | http://www.sciencedirect.com/science/article/pii/S2095311921637905 |
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