Combination of Isothermal Recombinase-Aided Amplification and CRISPR-Cas12a-Mediated Assay for Rapid Detection of Major Severe Acute Respiratory Syndrome Coronavirus 2 Variants of Concern

Combination of Isothermal Recombinase-Aided Amplification and CRISPR-Cas12a-Mediated Assay for Rapid Detection of Major Severe Acute Respiratory Syndrome Coronavirus 2 Variants of Concern

Coronavirus disease 2019 (COVID-19) pandemic caused by SARS-CoV-2 variants is a new and unsolved threat; therefore, it is an urgent and unmet need to develop a simple and rapid method for detecting and tracking SARS-CoV-2 variants. The spike gene of SARS-CoV-2 was amplified by isothermal recombinase...

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Bibliographic Details
Main Authors: Hongqing Lin, Yuanhao Liang, Lirong Zou, Baisheng Li, Jianhui Zhao, Haiying Wang, Jiufeng Sun, Xiaoling Deng, Shixing Tang
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-06-01
Series:Frontiers in Microbiology
Subjects:
SARS-CoV-2
isothermal amplification
CRISPR-Cas12a
variants of concern
variant genotyping
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2022.945133/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2022.945133/full

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