Identification of Extracellular DNA-Binding Proteins in the Biofilm Matrix

Identification of Extracellular DNA-Binding Proteins in the Biofilm Matrix

ABSTRACT We developed a new approach that couples Southwestern blotting and mass spectrometry to discover proteins that bind extracellular DNA (eDNA) in bacterial biofilms. Using Staphylococcus aureus as a model pathogen, we identified proteins with known DNA-binding activity and uncovered a series...

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Bibliographic Details
Main Authors: Jeffrey S. Kavanaugh, Caralyn E. Flack, Jessica Lister, Erica B. Ricker, Carolyn B. Ibberson, Christian Jenul, Derek E. Moormeier, Elizabeth A. Delmain, Kenneth W. Bayles, Alexander R. Horswill
Format: Article
Language:English
Published: American Society for Microbiology 2019-06-01
Series:mBio
Subjects:
MRSA
Southwestern blotting
Staphylococcus aureus
biofilms
eDNA
extracellular DNA
Online Access:https://journals.asm.org/doi/10.1128/mBio.01137-19

Internet

https://journals.asm.org/doi/10.1128/mBio.01137-19

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