Hemodynamic changes and neuronal damage detected by 9.4 T MRI in rats with chronic cerebral ischemia and cognitive impairment
Abstract Introduction The bilateral common carotid artery occlusion (BCCAO) rat model is an ideal animal model for simulating the pathology of chronic brain hypoperfusion in humans. However, dynamic changes in neuronal activity, cellular edema, and neuronal structural integrity in vivo after BCCAO h...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-07-01
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| Series: | Brain and Behavior |
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| Online Access: | https://doi.org/10.1002/brb3.2642 |
| _version_ | 1817971271852359680 |
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| author | Minghua Sun Liangmiao Wu Guangying Chen Xukai Mo Changzheng Shi |
| author_facet | Minghua Sun Liangmiao Wu Guangying Chen Xukai Mo Changzheng Shi |
| author_sort | Minghua Sun |
| collection | DOAJ |
| description | Abstract Introduction The bilateral common carotid artery occlusion (BCCAO) rat model is an ideal animal model for simulating the pathology of chronic brain hypoperfusion in humans. However, dynamic changes in neuronal activity, cellular edema, and neuronal structural integrity in vivo after BCCAO have rarely been reported. The purpose of this study is to use a 9.4 T MRI to explore the pathophysiological mechanisms of vascular dementia. Materials and Methods Twelve Sprague–Dawley (SD) rats were randomly divided into two groups: the sham group and the model group (n = 6 for each group). Rats were subjected to MRI using T2*WI, diffusion tensor imaging (DTI), and DWI sequences by MRI at the following six time points: presurgery and 6 h, 3 days, 7 days, 21 days, and 28 days postsurgery. Then, the T2*, fractional anisotropy (FA), and average apparent diffusion coefficient (ADC) values were measured in the bilateral cortices and hippocampi. After MRI scanning, all rats in both groups were subjected to the Y‐maze test, novel object recognition test, and open‐field test to assess their learning, memory, cognition, and locomotor activity. Results The T2*, FA, and ADC values in the cerebral cortex and hippocampus decreased sharply at 6 h after BCCAO in the model group compared with those of the sham group. By Day 28, the T2* and ADC values gradually increased to close to those in the sham group, but the FA values changed little, and the rats in the model group had worse learning, memory, and cognition and less locomotor activity than the rats in the sham group. Conclusions The BCCAO is an ideal rat model for studying the pathophysiological mechanisms of vascular dementia. |
| first_indexed | 2024-04-13T20:44:43Z |
| format | Article |
| id | doaj.art-a90e89fd1574494c91fbc11316b81371 |
| institution | Directory Open Access Journal |
| issn | 2162-3279 |
| language | English |
| last_indexed | 2024-04-13T20:44:43Z |
| publishDate | 2022-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Brain and Behavior |
| spelling | doaj.art-a90e89fd1574494c91fbc11316b813712022-12-22T02:30:44ZengWileyBrain and Behavior2162-32792022-07-01127n/an/a10.1002/brb3.2642Hemodynamic changes and neuronal damage detected by 9.4 T MRI in rats with chronic cerebral ischemia and cognitive impairmentMinghua Sun0Liangmiao Wu1Guangying Chen2Xukai Mo3Changzheng Shi4Medical Imaging Center The First Affiliated Hospital of Jinan University No 613 Huangpu Dadao West Guangzhou People's Republic of ChinaInstitute of New Drug Research, International Cooperative Laboratory of Traditional Chinese Medicine Moderation and innovative Drug Development of Chinese Ministry of Education Jinan University College of Pharmacy Guangzhou People's Republic of ChinaInstitute of New Drug Research, International Cooperative Laboratory of Traditional Chinese Medicine Moderation and innovative Drug Development of Chinese Ministry of Education Jinan University College of Pharmacy Guangzhou People's Republic of ChinaMedical Imaging Center The First Affiliated Hospital of Jinan University No 613 Huangpu Dadao West Guangzhou People's Republic of ChinaMedical Imaging Center The First Affiliated Hospital of Jinan University No 613 Huangpu Dadao West Guangzhou People's Republic of ChinaAbstract Introduction The bilateral common carotid artery occlusion (BCCAO) rat model is an ideal animal model for simulating the pathology of chronic brain hypoperfusion in humans. However, dynamic changes in neuronal activity, cellular edema, and neuronal structural integrity in vivo after BCCAO have rarely been reported. The purpose of this study is to use a 9.4 T MRI to explore the pathophysiological mechanisms of vascular dementia. Materials and Methods Twelve Sprague–Dawley (SD) rats were randomly divided into two groups: the sham group and the model group (n = 6 for each group). Rats were subjected to MRI using T2*WI, diffusion tensor imaging (DTI), and DWI sequences by MRI at the following six time points: presurgery and 6 h, 3 days, 7 days, 21 days, and 28 days postsurgery. Then, the T2*, fractional anisotropy (FA), and average apparent diffusion coefficient (ADC) values were measured in the bilateral cortices and hippocampi. After MRI scanning, all rats in both groups were subjected to the Y‐maze test, novel object recognition test, and open‐field test to assess their learning, memory, cognition, and locomotor activity. Results The T2*, FA, and ADC values in the cerebral cortex and hippocampus decreased sharply at 6 h after BCCAO in the model group compared with those of the sham group. By Day 28, the T2* and ADC values gradually increased to close to those in the sham group, but the FA values changed little, and the rats in the model group had worse learning, memory, and cognition and less locomotor activity than the rats in the sham group. Conclusions The BCCAO is an ideal rat model for studying the pathophysiological mechanisms of vascular dementia.https://doi.org/10.1002/brb3.2642bilateral common carotid artery occlusioncerebral hypoperfusiondementiamagnetic resonance diffusion tensor imageastrocyte |
| spellingShingle | Minghua Sun Liangmiao Wu Guangying Chen Xukai Mo Changzheng Shi Hemodynamic changes and neuronal damage detected by 9.4 T MRI in rats with chronic cerebral ischemia and cognitive impairment Brain and Behavior bilateral common carotid artery occlusion cerebral hypoperfusion dementia magnetic resonance diffusion tensor image astrocyte |
| title | Hemodynamic changes and neuronal damage detected by 9.4 T MRI in rats with chronic cerebral ischemia and cognitive impairment |
| title_full | Hemodynamic changes and neuronal damage detected by 9.4 T MRI in rats with chronic cerebral ischemia and cognitive impairment |
| title_fullStr | Hemodynamic changes and neuronal damage detected by 9.4 T MRI in rats with chronic cerebral ischemia and cognitive impairment |
| title_full_unstemmed | Hemodynamic changes and neuronal damage detected by 9.4 T MRI in rats with chronic cerebral ischemia and cognitive impairment |
| title_short | Hemodynamic changes and neuronal damage detected by 9.4 T MRI in rats with chronic cerebral ischemia and cognitive impairment |
| title_sort | hemodynamic changes and neuronal damage detected by 9 4 t mri in rats with chronic cerebral ischemia and cognitive impairment |
| topic | bilateral common carotid artery occlusion cerebral hypoperfusion dementia magnetic resonance diffusion tensor image astrocyte |
| url | https://doi.org/10.1002/brb3.2642 |
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