Albumin/Thiacalix[4]arene Nanoparticles as Potential Therapeutic Systems: Role of the Macrocycle for Stabilization of Monomeric Protein and Self-Assembly with Ciprofloxacin
The therapeutic application of serum albumin is determined by the relative content of the monomeric form compared to dimers, tetramers, hexamers, etc. In this paper, we propose and develop an approach to synthesize the <i>cone</i> stereoisomer of <i>p-tert</i>-butylthiacalix[...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/17/10040 |
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| author | Luidmila Yakimova Aisylu Kunafina Olga Mostovaya Pavel Padnya Timur Mukhametzyanov Alexandra Voloshina Konstantin Petrov Artur Boldyrev Ivan Stoikov |
| author_facet | Luidmila Yakimova Aisylu Kunafina Olga Mostovaya Pavel Padnya Timur Mukhametzyanov Alexandra Voloshina Konstantin Petrov Artur Boldyrev Ivan Stoikov |
| author_sort | Luidmila Yakimova |
| collection | DOAJ |
| description | The therapeutic application of serum albumin is determined by the relative content of the monomeric form compared to dimers, tetramers, hexamers, etc. In this paper, we propose and develop an approach to synthesize the <i>cone</i> stereoisomer of <i>p-tert</i>-butylthiacalix[4]arene with sulfobetaine fragments stabilization of monomeric bovine serum albumin and preventing aggregation. Spectral methods (UV-vis, CD, fluorescent spectroscopy, and dynamic light scattering) established the influence of the synthesized compounds on the content of monomeric and aggregated forms of BSA even without the formation of stable thiacalixarene/protein associates. The effect of thiacalixarenes on the efficiency of protein binding with the antibiotic ciprofloxacin was shown by fluorescence spectroscopy. The binding constant increases in the presence of the macrocycles, likely due to the stabilization of monomeric forms of BSA. Our study clearly shows the potential of this macrocycle design as a platform for the development of the fundamentally new approaches for preventing aggregation. |
| first_indexed | 2024-03-10T01:42:32Z |
| format | Article |
| id | doaj.art-a95f0db6b6be428e941a2d812cad1053 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T01:42:32Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a95f0db6b6be428e941a2d812cad10532023-11-23T13:22:03ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123171004010.3390/ijms231710040Albumin/Thiacalix[4]arene Nanoparticles as Potential Therapeutic Systems: Role of the Macrocycle for Stabilization of Monomeric Protein and Self-Assembly with CiprofloxacinLuidmila Yakimova0Aisylu Kunafina1Olga Mostovaya2Pavel Padnya3Timur Mukhametzyanov4Alexandra Voloshina5Konstantin Petrov6Artur Boldyrev7Ivan Stoikov8A.M. Butlerov Chemistry Institute, Kazan Federal University, 18 Kremlyovskaya Str., 420008 Kazan, RussiaA.M. Butlerov Chemistry Institute, Kazan Federal University, 18 Kremlyovskaya Str., 420008 Kazan, RussiaA.M. Butlerov Chemistry Institute, Kazan Federal University, 18 Kremlyovskaya Str., 420008 Kazan, RussiaA.M. Butlerov Chemistry Institute, Kazan Federal University, 18 Kremlyovskaya Str., 420008 Kazan, RussiaA.M. Butlerov Chemistry Institute, Kazan Federal University, 18 Kremlyovskaya Str., 420008 Kazan, RussiaArbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov Street, 420088 Kazan, RussiaArbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov Street, 420088 Kazan, RussiaA.M. Butlerov Chemistry Institute, Kazan Federal University, 18 Kremlyovskaya Str., 420008 Kazan, RussiaA.M. Butlerov Chemistry Institute, Kazan Federal University, 18 Kremlyovskaya Str., 420008 Kazan, RussiaThe therapeutic application of serum albumin is determined by the relative content of the monomeric form compared to dimers, tetramers, hexamers, etc. In this paper, we propose and develop an approach to synthesize the <i>cone</i> stereoisomer of <i>p-tert</i>-butylthiacalix[4]arene with sulfobetaine fragments stabilization of monomeric bovine serum albumin and preventing aggregation. Spectral methods (UV-vis, CD, fluorescent spectroscopy, and dynamic light scattering) established the influence of the synthesized compounds on the content of monomeric and aggregated forms of BSA even without the formation of stable thiacalixarene/protein associates. The effect of thiacalixarenes on the efficiency of protein binding with the antibiotic ciprofloxacin was shown by fluorescence spectroscopy. The binding constant increases in the presence of the macrocycles, likely due to the stabilization of monomeric forms of BSA. Our study clearly shows the potential of this macrocycle design as a platform for the development of the fundamentally new approaches for preventing aggregation.https://www.mdpi.com/1422-0067/23/17/10040sulfobetainesthiacalixareneself-assemblybovine serum albuminciprofloxacin |
| spellingShingle | Luidmila Yakimova Aisylu Kunafina Olga Mostovaya Pavel Padnya Timur Mukhametzyanov Alexandra Voloshina Konstantin Petrov Artur Boldyrev Ivan Stoikov Albumin/Thiacalix[4]arene Nanoparticles as Potential Therapeutic Systems: Role of the Macrocycle for Stabilization of Monomeric Protein and Self-Assembly with Ciprofloxacin International Journal of Molecular Sciences sulfobetaines thiacalixarene self-assembly bovine serum albumin ciprofloxacin |
| title | Albumin/Thiacalix[4]arene Nanoparticles as Potential Therapeutic Systems: Role of the Macrocycle for Stabilization of Monomeric Protein and Self-Assembly with Ciprofloxacin |
| title_full | Albumin/Thiacalix[4]arene Nanoparticles as Potential Therapeutic Systems: Role of the Macrocycle for Stabilization of Monomeric Protein and Self-Assembly with Ciprofloxacin |
| title_fullStr | Albumin/Thiacalix[4]arene Nanoparticles as Potential Therapeutic Systems: Role of the Macrocycle for Stabilization of Monomeric Protein and Self-Assembly with Ciprofloxacin |
| title_full_unstemmed | Albumin/Thiacalix[4]arene Nanoparticles as Potential Therapeutic Systems: Role of the Macrocycle for Stabilization of Monomeric Protein and Self-Assembly with Ciprofloxacin |
| title_short | Albumin/Thiacalix[4]arene Nanoparticles as Potential Therapeutic Systems: Role of the Macrocycle for Stabilization of Monomeric Protein and Self-Assembly with Ciprofloxacin |
| title_sort | albumin thiacalix 4 arene nanoparticles as potential therapeutic systems role of the macrocycle for stabilization of monomeric protein and self assembly with ciprofloxacin |
| topic | sulfobetaines thiacalixarene self-assembly bovine serum albumin ciprofloxacin |
| url | https://www.mdpi.com/1422-0067/23/17/10040 |
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