Seamless Coupling of Chemical Glycan Release and Labeling for an Accelerated Protein <i>N</i>-Glycan Sample Preparation Workflow

Seamless Coupling of Chemical Glycan Release and Labeling for an Accelerated Protein <i>N</i>-Glycan Sample Preparation Workflow

Analytical methods fr direct quantitative <i>N</i>-glycan analysis require a sequence of sample preparation and clean-up steps that result in reduced glycan recovery. Therefore, we aimed to combine glycan release and labeling steps. Based on the hypothesis that the reaction mechanism for...

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Bibliographic Details
Main Authors: Mumtaz Kasim, Anja Griebel, Grit Sandig, Robert Höltzel, Akshay Malhotra, Stephan Hinderlich, Volker Sandig, Barbara Müller, Hans Henning von Horsten
Format: Article
Language:English
Published: MDPI AG 2023-05-01
Series:Bioengineering
Subjects:
glycoproteins
glycan analytics
<i>N</i>-glycans
Hofmann rearrangement
glycan sample preparation
bioprocessing
Online Access:https://www.mdpi.com/2306-5354/10/6/651

Internet

https://www.mdpi.com/2306-5354/10/6/651

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