Hidrolisis beberapa jenis xilan dengan enzim xilanolitik termofilik rekombinan

Hidrolisis beberapa jenis xilan dengan enzim xilanolitik termofilik rekombinan

The aims of this research were to know the ability of recombinant xylanolytic enzyme from recombinant E. coli DH5a (pTP510) to hydrolyze several commercial xylan and analysis the reduction sugar product. Recombinant xylanolytic enzyme (exo-xylanase, b-xylosidase and a-L-arabinofuranosidase) could...

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Bibliographic Details
Main Authors: Ni Nyoman Tri Puspaningsih, Hery Suwito, Sri Sumarsih, Ali Rohman, One Asmarani
Format: Article
Language:English
Published: Indonesian Biological Society 2012-02-01
Series:Journal of Biological Researches
Subjects:
b-xylosidase and a-L-arabinofuranosidase) could hydrolyzed several commercial xylan (oat-spelt xylan
birchwood
wheat
rye
and arabinan) with xylanolytic activities are: oat-spelt xylan (1.73 U/mL)
birchwood (0.92 U/mL)
wheat (6.52 U/mL)
rye (4.94 U/mL)
and arabinan (3.40 U/mL). Xylanolytic enzyme assay use specific substrate p-nitrophenyl-b-D-xylopyranoside (pNP-X) shown xylosidase activity 15.869 U/mL. Hydrolysis product was analyzed by HPLC. The results showed that xylose
arabinose
and xylo-oligosaccharide were produced from birchwood
and arabinan hydrolysis
commercial xylan
pNP-X
pTP510
xylanolytic enzyme
Online Access:http://berkalahayati.org/journal/5ddc0f8c

Internet

http://berkalahayati.org/journal/5ddc0f8c

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