Protocol for the setup and use of a human choroid plexus endothelial-epithelial two-cell-type in vitro model

Summary: Choroid plexus, located in brain ventricles, is the site of blood-cerebrospinal fluid barrier that contains endothelial cells and an epithelial monolayer separated by stroma. We established a two-cell-type model of the human choroid plexus consisting of immortalized endothelial cells (iHCPE...

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Bibliographic Details
Main Authors: Rosanna Herold, Julia Borkowski, Christian Schwerk, Carolin Stump-Guthier, Marko Lampe, Hiroshi Ishikawa, Walter Muranyi, Horst Schroten
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722006967