Green Route for the Isolation and Purification of Hyrdoxytyrosol, Tyrosol, Oleacein and Oleocanthal from Extra Virgin Olive Oil
Extra virgin olive oil (EVOO) phenols represent a significant part of the intake of antioxidants and bioactive compounds in the Mediterranean diet. In particular, hydroxytyrosol (HTyr), tyrosol (Tyr), and the secoiridoids oleacein and oleocanthal play central roles as anti-inflammatory, neuro-protec...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/16/3654 |
| _version_ | 1827710426485358592 |
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| author | Antonio Francioso Rodolfo Federico Anna Maggiore Mario Fontana Alberto Boffi Maria D’Erme Luciana Mosca |
| author_facet | Antonio Francioso Rodolfo Federico Anna Maggiore Mario Fontana Alberto Boffi Maria D’Erme Luciana Mosca |
| author_sort | Antonio Francioso |
| collection | DOAJ |
| description | Extra virgin olive oil (EVOO) phenols represent a significant part of the intake of antioxidants and bioactive compounds in the Mediterranean diet. In particular, hydroxytyrosol (HTyr), tyrosol (Tyr), and the secoiridoids oleacein and oleocanthal play central roles as anti-inflammatory, neuro-protective and anti-cancer agents. These compounds cannot be easily obtained via chemical synthesis, and their isolation and purification from EVOO is cumbersome. Indeed, both processes involve the use of large volumes of organic solvents, hazardous reagents and several chromatographic steps. In this work we propose a novel optimized procedure for the green extraction, isolation and purification of HTyr, Tyr, oleacein and oleocanthal directly from EVOO, by using a Natural Deep Eutectic Solvent (NaDES) as an extracting phase, coupled with preparative high-performance liquid chromatography. This purification method allows the total recovery of the four components as single pure compounds directly from EVOO, in a rapid, economic and ecologically sustainable way, which utilizes biocompatible reagents and strongly limits the use or generation of hazardous substances. |
| first_indexed | 2024-03-10T17:38:52Z |
| format | Article |
| id | doaj.art-a9ee44452a7b43d2a62a946142a0a66c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T17:38:52Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a9ee44452a7b43d2a62a946142a0a66c2023-11-20T09:47:04ZengMDPI AGMolecules1420-30492020-08-012516365410.3390/molecules25163654Green Route for the Isolation and Purification of Hyrdoxytyrosol, Tyrosol, Oleacein and Oleocanthal from Extra Virgin Olive OilAntonio Francioso0Rodolfo Federico1Anna Maggiore2Mario Fontana3Alberto Boffi4Maria D’Erme5Luciana Mosca6Department of Biochemical Sciences, “Sapienza” University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyMOLIROM s.r.l, via Carlo Bartolomeo Piazza 8, 00161 Rome, ItalyDepartment of Biochemical Sciences, “Sapienza” University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyDepartment of Biochemical Sciences, “Sapienza” University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyDepartment of Biochemical Sciences, “Sapienza” University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyDepartment of Biochemical Sciences, “Sapienza” University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyDepartment of Biochemical Sciences, “Sapienza” University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyExtra virgin olive oil (EVOO) phenols represent a significant part of the intake of antioxidants and bioactive compounds in the Mediterranean diet. In particular, hydroxytyrosol (HTyr), tyrosol (Tyr), and the secoiridoids oleacein and oleocanthal play central roles as anti-inflammatory, neuro-protective and anti-cancer agents. These compounds cannot be easily obtained via chemical synthesis, and their isolation and purification from EVOO is cumbersome. Indeed, both processes involve the use of large volumes of organic solvents, hazardous reagents and several chromatographic steps. In this work we propose a novel optimized procedure for the green extraction, isolation and purification of HTyr, Tyr, oleacein and oleocanthal directly from EVOO, by using a Natural Deep Eutectic Solvent (NaDES) as an extracting phase, coupled with preparative high-performance liquid chromatography. This purification method allows the total recovery of the four components as single pure compounds directly from EVOO, in a rapid, economic and ecologically sustainable way, which utilizes biocompatible reagents and strongly limits the use or generation of hazardous substances.https://www.mdpi.com/1420-3049/25/16/3654extra virgin olive oiloleocanthalsecoiridoidsgreen chemistryhydoxytyrosoltyrosol |
| spellingShingle | Antonio Francioso Rodolfo Federico Anna Maggiore Mario Fontana Alberto Boffi Maria D’Erme Luciana Mosca Green Route for the Isolation and Purification of Hyrdoxytyrosol, Tyrosol, Oleacein and Oleocanthal from Extra Virgin Olive Oil Molecules extra virgin olive oil oleocanthal secoiridoids green chemistry hydoxytyrosol tyrosol |
| title | Green Route for the Isolation and Purification of Hyrdoxytyrosol, Tyrosol, Oleacein and Oleocanthal from Extra Virgin Olive Oil |
| title_full | Green Route for the Isolation and Purification of Hyrdoxytyrosol, Tyrosol, Oleacein and Oleocanthal from Extra Virgin Olive Oil |
| title_fullStr | Green Route for the Isolation and Purification of Hyrdoxytyrosol, Tyrosol, Oleacein and Oleocanthal from Extra Virgin Olive Oil |
| title_full_unstemmed | Green Route for the Isolation and Purification of Hyrdoxytyrosol, Tyrosol, Oleacein and Oleocanthal from Extra Virgin Olive Oil |
| title_short | Green Route for the Isolation and Purification of Hyrdoxytyrosol, Tyrosol, Oleacein and Oleocanthal from Extra Virgin Olive Oil |
| title_sort | green route for the isolation and purification of hyrdoxytyrosol tyrosol oleacein and oleocanthal from extra virgin olive oil |
| topic | extra virgin olive oil oleocanthal secoiridoids green chemistry hydoxytyrosol tyrosol |
| url | https://www.mdpi.com/1420-3049/25/16/3654 |
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