Inducing primary brainstem gliomas in genetically engineered mice using RCAS/TVA retroviruses and Cre/loxP recombination
Summary: Genetically engineered mice are commonly used to model brainstem gliomas in pre-clinical research. One technique for inducing primary tumors in these genetically engineered mice involves delivering viral vectors containing the code for gene-editing proteins. We present a protocol for genera...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723000527 |
| _version_ | 1811164940411600896 |
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| author | Loren B. Weidenhammer Harrison Q. Liu Lixia Luo Nerissa T. Williams Katherine Deland David G. Kirsch Zachary J. Reitman |
| author_facet | Loren B. Weidenhammer Harrison Q. Liu Lixia Luo Nerissa T. Williams Katherine Deland David G. Kirsch Zachary J. Reitman |
| author_sort | Loren B. Weidenhammer |
| collection | DOAJ |
| description | Summary: Genetically engineered mice are commonly used to model brainstem gliomas in pre-clinical research. One technique for inducing primary tumors in these genetically engineered mice involves delivering viral vectors containing the code for gene-editing proteins. We present a protocol for generating primary brainstem gliomas using the RCAS-TVA retroviral delivery system and the Cre/loxP gene editing system. We describe steps for transfecting and harvesting chicken fibroblast cells, intracranially injecting cells into mice, imaging primary tumors, and treating primary tumors with focal, image-guided brain irradiation.For complete details on the use and execution of this protocol, please refer to Deland et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-10T15:29:24Z |
| format | Article |
| id | doaj.art-a9f6e475ea374e718a6b2de094d7ebb5 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-10T15:29:24Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-a9f6e475ea374e718a6b2de094d7ebb52023-02-14T04:07:16ZengElsevierSTAR Protocols2666-16672023-03-0141102094Inducing primary brainstem gliomas in genetically engineered mice using RCAS/TVA retroviruses and Cre/loxP recombinationLoren B. Weidenhammer0Harrison Q. Liu1Lixia Luo2Nerissa T. Williams3Katherine Deland4David G. Kirsch5Zachary J. Reitman6Department of Radiation Oncology, Duke University School of Medicine, Durham, NC 27701, USA; Corresponding authorDepartment of Radiation Oncology, Duke University School of Medicine, Durham, NC 27701, USADepartment of Radiation Oncology, Duke University School of Medicine, Durham, NC 27701, USADepartment of Radiation Oncology, Duke University School of Medicine, Durham, NC 27701, USAOffice of Regulatory Affairs and Quality, Duke University School of Medicine, Durham, NC 27701, USADepartment of Radiation Oncology, Duke University School of Medicine, Durham, NC 27701, USA; Department of Pharmacology & Cancer Biology, Duke University School of Medicine, Durham, NC 27701, USADepartment of Radiation Oncology, Duke University School of Medicine, Durham, NC 27701, USA; Department of Pathology, Duke University School of Medicine, Durham, NC 27701, USA; Department of Neurosurgery, Duke University School of Medicine, Durham, NC 27701, USA; The Preston Robert Tisch Brain Tumor Center at Duke, Duke University School of Medicine, Durham, NC 27701, USA; Corresponding authorSummary: Genetically engineered mice are commonly used to model brainstem gliomas in pre-clinical research. One technique for inducing primary tumors in these genetically engineered mice involves delivering viral vectors containing the code for gene-editing proteins. We present a protocol for generating primary brainstem gliomas using the RCAS-TVA retroviral delivery system and the Cre/loxP gene editing system. We describe steps for transfecting and harvesting chicken fibroblast cells, intracranially injecting cells into mice, imaging primary tumors, and treating primary tumors with focal, image-guided brain irradiation.For complete details on the use and execution of this protocol, please refer to Deland et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723000527CancerHealth SciencesMicroscopyModel OrganismsMolecular BiologyNeuroscience |
| spellingShingle | Loren B. Weidenhammer Harrison Q. Liu Lixia Luo Nerissa T. Williams Katherine Deland David G. Kirsch Zachary J. Reitman Inducing primary brainstem gliomas in genetically engineered mice using RCAS/TVA retroviruses and Cre/loxP recombination STAR Protocols Cancer Health Sciences Microscopy Model Organisms Molecular Biology Neuroscience |
| title | Inducing primary brainstem gliomas in genetically engineered mice using RCAS/TVA retroviruses and Cre/loxP recombination |
| title_full | Inducing primary brainstem gliomas in genetically engineered mice using RCAS/TVA retroviruses and Cre/loxP recombination |
| title_fullStr | Inducing primary brainstem gliomas in genetically engineered mice using RCAS/TVA retroviruses and Cre/loxP recombination |
| title_full_unstemmed | Inducing primary brainstem gliomas in genetically engineered mice using RCAS/TVA retroviruses and Cre/loxP recombination |
| title_short | Inducing primary brainstem gliomas in genetically engineered mice using RCAS/TVA retroviruses and Cre/loxP recombination |
| title_sort | inducing primary brainstem gliomas in genetically engineered mice using rcas tva retroviruses and cre loxp recombination |
| topic | Cancer Health Sciences Microscopy Model Organisms Molecular Biology Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723000527 |
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