Active Expression of Human Hyaluronidase PH20 and Characterization of Its Hydrolysis Pattern

Active Expression of Human Hyaluronidase PH20 and Characterization of Its Hydrolysis Pattern

Hyaluronidases are a group of glycosidases catalyzing the degradation of hyaluronic acid (HA). Because of the advantages of effectively hydrolyzing the HA-rich matrix and low immunogenicity, human hyaluronidase PH20 (hPH20) is widely used in the medical field. Here, we realized the active expression...

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Bibliographic Details
Main Authors: Bo Pang, Jing He, Weijiao Zhang, Hao Huang, Yang Wang, Miao Wang, Guocheng Du, Zhen Kang
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-05-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
Pichia pastoris
human hyaluronidase
PH20
domain truncation
heterologous expression
hydrolysis pattern
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2022.885888/full

Internet

https://www.frontiersin.org/articles/10.3389/fbioe.2022.885888/full

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