An optimized protocol for in vitro and in cellulo structural determination of the multi-tRNA synthetase complex by cross-linking mass spectrometry

An optimized protocol for in vitro and in cellulo structural determination of the multi-tRNA synthetase complex by cross-linking mass spectrometry

Summary: Despite recent advances in structural determination of individual proteins, elucidating the 3-dimensional architecture of large, multiprotein complexes remains challenging, partly because of issues related to structural integrity during purification. Here, we describe a protocol to determin...

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Bibliographic Details
Main Authors: Krishnendu Khan, Camelia Baleanu-Gogonea, Belinda Willard, Valentin Gogonea, Paul L. Fox
Format: Article
Language:English
Published: Elsevier 2022-03-01
Series:STAR Protocols
Subjects:
Cell Biology
Molecular Biology
Protein Biochemistry
Structural Biology
Mass Spectrometry
Chemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722000818

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http://www.sciencedirect.com/science/article/pii/S2666166722000818

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