Nested reverse transcriptase–polymerase chain reactions targeting the messenger RNA of icl2, hspx, and rRNAP1 genes to detect viable Mycobacterium tuberculosis directly from clinical specimens

There is an urgent need for a rapid and reliable test to detect actively multiplying Mycobacterium tuberculosis directly from clinical specimens for an early initiation of the appropriate antituberculous treatment. This study was aimed at the optimization and application of nested reverse transcript...

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Bibliographic Details
Main Authors: Dhanurekha Lakshmipathy, Lily Therese Kulandai, Gayathri Ramasubban, Madhavan Hajib Narahari Rao, Sridhar Rathinam, Meenakshi Narasimhan
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2015-01-01
Series:International Journal of Mycobacteriology
Subjects:
Online Access:http://www.ijmyco.org/article.asp?issn=2212-5531;year=2015;volume=4;issue=4;spage=312;epage=317;aulast=Lakshmipathy