Balancing read length and sequencing depth: Optimizing Nanopore long‐read sequencing for monocots with an emphasis on the Liliales

Balancing read length and sequencing depth: Optimizing Nanopore long‐read sequencing for monocots with an emphasis on the Liliales

Abstract Premise We present approaches used to generate long‐read Nanopore sequencing reads for the Liliales and demonstrate how modifications to standard protocols directly impact read length and total output. The goal is to help those interested in generating long‐read sequencing data determine wh...

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Bibliographic Details
Main Authors: Gisel Y. De La Cerda, Jacob B. Landis, Evan Eifler, Adriana I. Hernandez, Fay‐Wei Li, Jing Zhang, Carrie M. Tribble, Nisa Karimi, Patricia Chan, Thomas Givnish, Susan R. Strickler, Chelsea D. Specht
Format: Article
Language:English
Published: Wiley 2023-05-01
Series:Applications in Plant Sciences
Subjects:
Calochortus
genome sequencing
Liliaceae
MinION
N50
Oxford Nanopore
Online Access:https://doi.org/10.1002/aps3.11524

Internet

https://doi.org/10.1002/aps3.11524

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