N-Butanol Extract of Modified You-Gui-Yin Attenuates Osteoclastogenesis and Ameliorates Osteoporosis by Inhibiting RANKL-Mediated NF-κB Signaling
Postmenopausal Osteoporosis (PMOP) is the most prevalent primary osteoporosis, attributable to an imbalance in osteoblast and osteoclast activity. Modified You-Gui-Yin (MYGY), a traditional Chinese herbal formula, is able to effectively treat PMOP, while the critical components and pharmacological m...
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| Format: | Article |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2022.925848/full |
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| author | Qinghe Zeng Qinghe Zeng Rui Xu Houfu Ling Shan Zhao Xu Wang Wenhua Yuan Mancang Gu Taotao Xu Pinger Wang Hongfeng Ruan Hongting Jin Hangbo Qu Fusheng Ye Jiali Chen |
| author_facet | Qinghe Zeng Qinghe Zeng Rui Xu Houfu Ling Shan Zhao Xu Wang Wenhua Yuan Mancang Gu Taotao Xu Pinger Wang Hongfeng Ruan Hongting Jin Hangbo Qu Fusheng Ye Jiali Chen |
| author_sort | Qinghe Zeng |
| collection | DOAJ |
| description | Postmenopausal Osteoporosis (PMOP) is the most prevalent primary osteoporosis, attributable to an imbalance in osteoblast and osteoclast activity. Modified You-Gui-Yin (MYGY), a traditional Chinese herbal formula, is able to effectively treat PMOP, while the critical components and pharmacological mechanisms of MYGY are still unclear. In this study, we aimed to investigate the therapeutic effects and underlying mechanisms of N-butanol extract of MYGY (MYGY-Nb) in ovariectomized (OVX)-induced osteoporosis mice. Histological staining and micro-computed tomography (μCT) analysis showed that MYGY-Nb was more effective in the suppression of OVX-induced bone loss than MYGY original formula. Subsequently, liquid chromatography and mass spectrometry analysis identified 16 critical compounds of MYGY-Nb and some of them are reported to affect osteoclast functions. Furthermore, in vivo and in vitro experiments demonstrated that MYGY-Nb significantly attenuated osteoclastogenesis by down-regulating RANKL-mediated NF-κB signaling. In conclusion, our study indicated that MYGY-Nb suppresses NF-κB signaling and osteoclast formation to mitigate bone loss in PMOP, implying that MYGY-Nb and its compounds are potential candidates for development of anti-PMOP drugs. |
| first_indexed | 2024-04-13T19:24:49Z |
| format | Article |
| id | doaj.art-aad52abee5d44ecabd4a4a45d053f72f |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-13T19:24:49Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-aad52abee5d44ecabd4a4a45d053f72f2022-12-22T02:33:24ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922022-06-011310.3389/fendo.2022.925848925848N-Butanol Extract of Modified You-Gui-Yin Attenuates Osteoclastogenesis and Ameliorates Osteoporosis by Inhibiting RANKL-Mediated NF-κB SignalingQinghe Zeng0Qinghe Zeng1Rui Xu2Houfu Ling3Shan Zhao4Xu Wang5Wenhua Yuan6Mancang Gu7Taotao Xu8Pinger Wang9Hongfeng Ruan10Hongting Jin11Hangbo Qu12Fusheng Ye13Jiali Chen14Institute of Orthopedics and Traumatology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe First College of Clinical Medicine, Zhejiang Chinese Medical University, Hangzhou, ChinaDepartment of Orthopaedics, Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang, ChinaDepartment of Orthopaedics, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, ChinaInstitute of Orthopedics and Traumatology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaInstitute of Orthopedics and Traumatology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, ChinaDepartment of Orthopaedics, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaInstitute of Orthopedics and Traumatology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaInstitute of Orthopedics and Traumatology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaInstitute of Orthopedics and Traumatology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaDepartment of Orthopaedic Surgery, Zhejiang Hospital, Hangzhou, ChinaAffiliated Xiaoshan Hospital, Hangzhou Normal University, Hangzhou, ChinaInstitute of Orthopedics and Traumatology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaPostmenopausal Osteoporosis (PMOP) is the most prevalent primary osteoporosis, attributable to an imbalance in osteoblast and osteoclast activity. Modified You-Gui-Yin (MYGY), a traditional Chinese herbal formula, is able to effectively treat PMOP, while the critical components and pharmacological mechanisms of MYGY are still unclear. In this study, we aimed to investigate the therapeutic effects and underlying mechanisms of N-butanol extract of MYGY (MYGY-Nb) in ovariectomized (OVX)-induced osteoporosis mice. Histological staining and micro-computed tomography (μCT) analysis showed that MYGY-Nb was more effective in the suppression of OVX-induced bone loss than MYGY original formula. Subsequently, liquid chromatography and mass spectrometry analysis identified 16 critical compounds of MYGY-Nb and some of them are reported to affect osteoclast functions. Furthermore, in vivo and in vitro experiments demonstrated that MYGY-Nb significantly attenuated osteoclastogenesis by down-regulating RANKL-mediated NF-κB signaling. In conclusion, our study indicated that MYGY-Nb suppresses NF-κB signaling and osteoclast formation to mitigate bone loss in PMOP, implying that MYGY-Nb and its compounds are potential candidates for development of anti-PMOP drugs.https://www.frontiersin.org/articles/10.3389/fendo.2022.925848/fullN-butanol extractionModified You-Gui-Yinpostmenopausal osteoporosisosteoclast formationNF-κB pathway |
| spellingShingle | Qinghe Zeng Qinghe Zeng Rui Xu Houfu Ling Shan Zhao Xu Wang Wenhua Yuan Mancang Gu Taotao Xu Pinger Wang Hongfeng Ruan Hongting Jin Hangbo Qu Fusheng Ye Jiali Chen N-Butanol Extract of Modified You-Gui-Yin Attenuates Osteoclastogenesis and Ameliorates Osteoporosis by Inhibiting RANKL-Mediated NF-κB Signaling Frontiers in Endocrinology N-butanol extraction Modified You-Gui-Yin postmenopausal osteoporosis osteoclast formation NF-κB pathway |
| title | N-Butanol Extract of Modified You-Gui-Yin Attenuates Osteoclastogenesis and Ameliorates Osteoporosis by Inhibiting RANKL-Mediated NF-κB Signaling |
| title_full | N-Butanol Extract of Modified You-Gui-Yin Attenuates Osteoclastogenesis and Ameliorates Osteoporosis by Inhibiting RANKL-Mediated NF-κB Signaling |
| title_fullStr | N-Butanol Extract of Modified You-Gui-Yin Attenuates Osteoclastogenesis and Ameliorates Osteoporosis by Inhibiting RANKL-Mediated NF-κB Signaling |
| title_full_unstemmed | N-Butanol Extract of Modified You-Gui-Yin Attenuates Osteoclastogenesis and Ameliorates Osteoporosis by Inhibiting RANKL-Mediated NF-κB Signaling |
| title_short | N-Butanol Extract of Modified You-Gui-Yin Attenuates Osteoclastogenesis and Ameliorates Osteoporosis by Inhibiting RANKL-Mediated NF-κB Signaling |
| title_sort | n butanol extract of modified you gui yin attenuates osteoclastogenesis and ameliorates osteoporosis by inhibiting rankl mediated nf κb signaling |
| topic | N-butanol extraction Modified You-Gui-Yin postmenopausal osteoporosis osteoclast formation NF-κB pathway |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2022.925848/full |
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