One pattern analysis (OPA) for the quantitative determination of protein interactions in plant cells
Abstract Background A commonly used approach to study the interaction of two proteins of interest (POIs) in vivo is measuring Förster Resonance Energy Transfer (FRET). This requires the expression of the two POIs fused to two fluorescent proteins that function as a FRET pair. A precise way to record...
Main Authors: | , , , , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2023-07-01
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Series: | Plant Methods |
Online Access: | https://doi.org/10.1186/s13007-023-01049-3 |