One pattern analysis (OPA) for the quantitative determination of protein interactions in plant cells

Abstract Background A commonly used approach to study the interaction of two proteins of interest (POIs) in vivo is measuring Förster Resonance Energy Transfer (FRET). This requires the expression of the two POIs fused to two fluorescent proteins that function as a FRET pair. A precise way to record...

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Bibliographic Details
Main Authors: Jan Eric Maika, Benedikt Krämer, Vivien I. Strotmann, Frank Wellmer, Stefanie Weidtkamp-Peters, Yvonne Stahl, Rüdiger Simon
Format: Article
Language:English
Published: BMC 2023-07-01
Series:Plant Methods
Online Access:https://doi.org/10.1186/s13007-023-01049-3