Human MxA is a potent interspecies barrier for the novel bat-derived influenza A-like virus H18N11
ABSTRACTThe human innate immune factor MxA represents an effective interspecies barrier for zoonotic influenza A viruses (IAVs) of animal origin. Accordingly, human but not avian IAVs efficiently escape the antiviral activity of MxA due to adaptive mutations in their viral nucleoprotein. Partial MxA...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Emerging Microbes and Infections |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2019.1599301 |
| _version_ | 1797385783731027968 |
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| author | Kevin Ciminski Johanna Pulvermüller Julia Adam Martin Schwemmle |
| author_facet | Kevin Ciminski Johanna Pulvermüller Julia Adam Martin Schwemmle |
| author_sort | Kevin Ciminski |
| collection | DOAJ |
| description | ABSTRACTThe human innate immune factor MxA represents an effective interspecies barrier for zoonotic influenza A viruses (IAVs) of animal origin. Accordingly, human but not avian IAVs efficiently escape the antiviral activity of MxA due to adaptive mutations in their viral nucleoprotein. Partial MxA resistance can be acquired in intermediate hosts such as swine, which possess an antivirally active Mx1 protein. Intriguingly, Mx1 of the bat Carollia perspicillata, a host of the recently discovered bat influenza A-like virus H18N11, is antivirally active against avian IAVs, thus raising the question whether H18N11 has undergone a preadaptation to human MxA. Here, by utilizing a chimeric bat influenza virus, PR8-H18N11, we demonstrate that MxA efficiently blocks viral replication in vitro as well as in MxA transgenic mice. Nevertheless, the H18N11 nucleoprotein exhibits partial MxA resistance in a polymerase reconstitution assay, suggesting that a certain degree of MxA preadaptation occurred. Together, our data indicate a currently reduced risk for H18N11 to overcome the human restriction factor MxA. Further adaptive mutations in NP are required to facilitate full MxA escape. |
| first_indexed | 2024-03-08T21:59:15Z |
| format | Article |
| id | doaj.art-aae968f5fef84e619ce5310be3c7424a |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-03-08T21:59:15Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-aae968f5fef84e619ce5310be3c7424a2023-12-19T16:09:57ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512019-01-018155656310.1080/22221751.2019.1599301Human MxA is a potent interspecies barrier for the novel bat-derived influenza A-like virus H18N11Kevin Ciminski0Johanna Pulvermüller1Julia Adam2Martin Schwemmle3Institute of Virology, Medical Center – University of Freiburg, Freiburg, GermanyInstitute of Virology, Medical Center – University of Freiburg, Freiburg, GermanyInstitute of Virology, Medical Center – University of Freiburg, Freiburg, GermanyInstitute of Virology, Medical Center – University of Freiburg, Freiburg, GermanyABSTRACTThe human innate immune factor MxA represents an effective interspecies barrier for zoonotic influenza A viruses (IAVs) of animal origin. Accordingly, human but not avian IAVs efficiently escape the antiviral activity of MxA due to adaptive mutations in their viral nucleoprotein. Partial MxA resistance can be acquired in intermediate hosts such as swine, which possess an antivirally active Mx1 protein. Intriguingly, Mx1 of the bat Carollia perspicillata, a host of the recently discovered bat influenza A-like virus H18N11, is antivirally active against avian IAVs, thus raising the question whether H18N11 has undergone a preadaptation to human MxA. Here, by utilizing a chimeric bat influenza virus, PR8-H18N11, we demonstrate that MxA efficiently blocks viral replication in vitro as well as in MxA transgenic mice. Nevertheless, the H18N11 nucleoprotein exhibits partial MxA resistance in a polymerase reconstitution assay, suggesting that a certain degree of MxA preadaptation occurred. Together, our data indicate a currently reduced risk for H18N11 to overcome the human restriction factor MxA. Further adaptive mutations in NP are required to facilitate full MxA escape.https://www.tandfonline.com/doi/10.1080/22221751.2019.1599301Bat influenza A virusH18N11chimeric influenza A virusPR8-H18N11MxAinterspecies barrier |
| spellingShingle | Kevin Ciminski Johanna Pulvermüller Julia Adam Martin Schwemmle Human MxA is a potent interspecies barrier for the novel bat-derived influenza A-like virus H18N11 Emerging Microbes and Infections Bat influenza A virus H18N11 chimeric influenza A virus PR8-H18N11 MxA interspecies barrier |
| title | Human MxA is a potent interspecies barrier for the novel bat-derived influenza A-like virus H18N11 |
| title_full | Human MxA is a potent interspecies barrier for the novel bat-derived influenza A-like virus H18N11 |
| title_fullStr | Human MxA is a potent interspecies barrier for the novel bat-derived influenza A-like virus H18N11 |
| title_full_unstemmed | Human MxA is a potent interspecies barrier for the novel bat-derived influenza A-like virus H18N11 |
| title_short | Human MxA is a potent interspecies barrier for the novel bat-derived influenza A-like virus H18N11 |
| title_sort | human mxa is a potent interspecies barrier for the novel bat derived influenza a like virus h18n11 |
| topic | Bat influenza A virus H18N11 chimeric influenza A virus PR8-H18N11 MxA interspecies barrier |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2019.1599301 |
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