Exogenous Application of Proline and L-Cysteine Alleviates Internal Browning and Maintains Eating Quality of Cold Stored Flat ‘Maleki’ Peach Fruits
The postharvest life of flat peach fruit is limited by the appearance of chilling injury symptoms, especially internal browning. In this study, impacts of the exogenous application of proline (0, 5, 10, and 15 mM) and L-cysteine (0, 0.2, 0.4 and 0.6%) on attenuating chilling injury of flat peach fru...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/7/11/469 |
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| author | Gholamreza Gohari Sanaz Molaei Azizollah Kheiry Mahshid Ghafouri Farhang Razavi Jose M. Lorenzo Antonio Juárez-Maldonado |
| author_facet | Gholamreza Gohari Sanaz Molaei Azizollah Kheiry Mahshid Ghafouri Farhang Razavi Jose M. Lorenzo Antonio Juárez-Maldonado |
| author_sort | Gholamreza Gohari |
| collection | DOAJ |
| description | The postharvest life of flat peach fruit is limited by the appearance of chilling injury symptoms, especially internal browning. In this study, impacts of the exogenous application of proline (0, 5, 10, and 15 mM) and L-cysteine (0, 0.2, 0.4 and 0.6%) on attenuating chilling injury of flat peach fruit were evaluated all over the cold storage. The results demonstrated that the fruits treated with 15 mM proline and 0.4 % L-cysteine showed lower levels of internal browning and these treatments prevented the excess enhancement of total soluble solids (TSS), the decline of titratable acidity (TA) content and the loss of fruit firmness during storage time. A lower accumulation of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), malondialdehyde (MDA), electrolyte leakage, and higher activity of antioxidant enzymes, along with higher ascorbic acid content and antioxidant capacity, were observed in treated fruits. Treated fruits also showed higher activity of phenylalanine ammonia lyase (PAL) and conversely lower activity of polyphenol oxidase (PPO), which led to a higher accumulation of total phenols and flavonoids. Moreover, a higher accumulation of endogenous proline was observed in 15 mM proline treated fruits. Eventually, according to our results, the exogenous administration of proline and L-cysteine as safe, natural and environmentally friendly treatments, preserved the nutritional quality of flat peach fruits during long-term cold storage. |
| first_indexed | 2024-03-10T05:26:49Z |
| format | Article |
| id | doaj.art-aaec24e8a32e410aa1efdb17e9fc207c |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T05:26:49Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-aaec24e8a32e410aa1efdb17e9fc207c2023-11-22T23:34:09ZengMDPI AGHorticulturae2311-75242021-11-0171146910.3390/horticulturae7110469Exogenous Application of Proline and L-Cysteine Alleviates Internal Browning and Maintains Eating Quality of Cold Stored Flat ‘Maleki’ Peach FruitsGholamreza Gohari0Sanaz Molaei1Azizollah Kheiry2Mahshid Ghafouri3Farhang Razavi4Jose M. Lorenzo5Antonio Juárez-Maldonado6Department of Horticulture, Faculty of Agriculture, University of Maragheh, Maragheh 83111-55181, IranDepartment of Horticulture, Faculty of Agriculture, University of Zanjan, Zanjan 45371-38791, IranDepartment of Horticulture, Faculty of Agriculture, University of Zanjan, Zanjan 45371-38791, IranDepartment of Horticulture, Faculty of Agriculture, University of Zanjan, Zanjan 45371-38791, IranDepartment of Horticulture, Faculty of Agriculture, University of Zanjan, Zanjan 45371-38791, IranCentro Tecnológico de la Carne de Galicia, Avd. Galicia n°4, Parque Tecnológico de Galicia, San Cibrao das Viñas, 32900 Ourense, SpainDepartamento de Botánica, Universidad Autónoma Agraria Antonio Narro, Saltillo 25315, Coahuila, MexicoThe postharvest life of flat peach fruit is limited by the appearance of chilling injury symptoms, especially internal browning. In this study, impacts of the exogenous application of proline (0, 5, 10, and 15 mM) and L-cysteine (0, 0.2, 0.4 and 0.6%) on attenuating chilling injury of flat peach fruit were evaluated all over the cold storage. The results demonstrated that the fruits treated with 15 mM proline and 0.4 % L-cysteine showed lower levels of internal browning and these treatments prevented the excess enhancement of total soluble solids (TSS), the decline of titratable acidity (TA) content and the loss of fruit firmness during storage time. A lower accumulation of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), malondialdehyde (MDA), electrolyte leakage, and higher activity of antioxidant enzymes, along with higher ascorbic acid content and antioxidant capacity, were observed in treated fruits. Treated fruits also showed higher activity of phenylalanine ammonia lyase (PAL) and conversely lower activity of polyphenol oxidase (PPO), which led to a higher accumulation of total phenols and flavonoids. Moreover, a higher accumulation of endogenous proline was observed in 15 mM proline treated fruits. Eventually, according to our results, the exogenous administration of proline and L-cysteine as safe, natural and environmentally friendly treatments, preserved the nutritional quality of flat peach fruits during long-term cold storage.https://www.mdpi.com/2311-7524/7/11/469antioxidant capacitymembrane integritypolyphenol oxidasepostharvest life<i>Prunus persica</i> |
| spellingShingle | Gholamreza Gohari Sanaz Molaei Azizollah Kheiry Mahshid Ghafouri Farhang Razavi Jose M. Lorenzo Antonio Juárez-Maldonado Exogenous Application of Proline and L-Cysteine Alleviates Internal Browning and Maintains Eating Quality of Cold Stored Flat ‘Maleki’ Peach Fruits Horticulturae antioxidant capacity membrane integrity polyphenol oxidase postharvest life <i>Prunus persica</i> |
| title | Exogenous Application of Proline and L-Cysteine Alleviates Internal Browning and Maintains Eating Quality of Cold Stored Flat ‘Maleki’ Peach Fruits |
| title_full | Exogenous Application of Proline and L-Cysteine Alleviates Internal Browning and Maintains Eating Quality of Cold Stored Flat ‘Maleki’ Peach Fruits |
| title_fullStr | Exogenous Application of Proline and L-Cysteine Alleviates Internal Browning and Maintains Eating Quality of Cold Stored Flat ‘Maleki’ Peach Fruits |
| title_full_unstemmed | Exogenous Application of Proline and L-Cysteine Alleviates Internal Browning and Maintains Eating Quality of Cold Stored Flat ‘Maleki’ Peach Fruits |
| title_short | Exogenous Application of Proline and L-Cysteine Alleviates Internal Browning and Maintains Eating Quality of Cold Stored Flat ‘Maleki’ Peach Fruits |
| title_sort | exogenous application of proline and l cysteine alleviates internal browning and maintains eating quality of cold stored flat maleki peach fruits |
| topic | antioxidant capacity membrane integrity polyphenol oxidase postharvest life <i>Prunus persica</i> |
| url | https://www.mdpi.com/2311-7524/7/11/469 |
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