A Glycosaminoglycan-Rich Fraction from Sea Cucumber <i>Isostichopus badionotus</i> Has Potent Anti-Inflammatory Properties In Vitro and In Vivo
Sea cucumber body wall contains several naturally occurring bioactive components that possess health-promoting properties. <i>Isostichopus badionotus</i> from Yucatan, Mexico is heavily fished, but little is known about its bioactive constituents. We previously established that <i>...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-06-01
|
| Series: | Nutrients |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6643/12/6/1698 |
| _version_ | 1797565951854510080 |
|---|---|
| author | Leticia Olivera-Castillo George Grant Nuvia Kantún-Moreno Hirian A. Barrera-Pérez Jorge Montero Miguel A. Olvera-Novoa Leydi M. Carrillo-Cocom Juan J. Acevedo Cesar Puerto-Castillo Victor May Solís Juan A. Pérez-Vega Judit Gil-Zamorano Enrique Hernández-Garibay María A. Fernández-Herrera Mayra Pérez-Tapia Oscar Medina-Contreras Jairo R. Villanueva-Toledo Rossanna Rodriguez-Canul Alberto Dávalos |
| author_facet | Leticia Olivera-Castillo George Grant Nuvia Kantún-Moreno Hirian A. Barrera-Pérez Jorge Montero Miguel A. Olvera-Novoa Leydi M. Carrillo-Cocom Juan J. Acevedo Cesar Puerto-Castillo Victor May Solís Juan A. Pérez-Vega Judit Gil-Zamorano Enrique Hernández-Garibay María A. Fernández-Herrera Mayra Pérez-Tapia Oscar Medina-Contreras Jairo R. Villanueva-Toledo Rossanna Rodriguez-Canul Alberto Dávalos |
| author_sort | Leticia Olivera-Castillo |
| collection | DOAJ |
| description | Sea cucumber body wall contains several naturally occurring bioactive components that possess health-promoting properties. <i>Isostichopus badionotus</i> from Yucatan, Mexico is heavily fished, but little is known about its bioactive constituents. We previously established that <i>I. badionotus</i> meal had potent anti-inflammatory properties in vivo. We have now screened some of its constituents for anti-inflammatory activity in vitro. Glycosaminoglycan and soluble protein preparations reduced 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammatory responses in HaCaT cells while an ethanol extract had a limited effect. The primary glycosaminoglycan (fucosylated chondroitin sulfate; FCS) was purified and tested for anti-inflammatory activity in vivo. FCS modulated the expression of critical genes, including NF-ĸB, TNFα, iNOS, and COX-2, and attenuated inflammation and tissue damage caused by TPA in a mouse ear inflammation model. It also mitigated colonic colitis caused in mice by dextran sodium sulfate. FCS from <i>I. badionotus</i> of the Yucatan Peninsula thus had strong anti-inflammatory properties in vivo. |
| first_indexed | 2024-03-10T19:20:07Z |
| format | Article |
| id | doaj.art-ab1a1d4c42604b76bdfba04f67db5486 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T19:20:07Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-ab1a1d4c42604b76bdfba04f67db54862023-11-20T03:03:22ZengMDPI AGNutrients2072-66432020-06-01126169810.3390/nu12061698A Glycosaminoglycan-Rich Fraction from Sea Cucumber <i>Isostichopus badionotus</i> Has Potent Anti-Inflammatory Properties In Vitro and In VivoLeticia Olivera-Castillo0George Grant1Nuvia Kantún-Moreno2Hirian A. Barrera-Pérez3Jorge Montero4Miguel A. Olvera-Novoa5Leydi M. Carrillo-Cocom6Juan J. Acevedo7Cesar Puerto-Castillo8Victor May Solís9Juan A. Pérez-Vega10Judit Gil-Zamorano11Enrique Hernández-Garibay12María A. Fernández-Herrera13Mayra Pérez-Tapia14Oscar Medina-Contreras15Jairo R. Villanueva-Toledo16Rossanna Rodriguez-Canul17Alberto Dávalos18Departamento Recursos del Mar, Centro de Investigación y de Estudios Avanzados del IPN-Unidad Mérida, Antigua Carretera a Progreso Km. 6, Merida 97310, Yucatan, MexicoSchool of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen AB25 2ZD, UKDepartamento Recursos del Mar, Centro de Investigación y de Estudios Avanzados del IPN-Unidad Mérida, Antigua Carretera a Progreso Km. 6, Merida 97310, Yucatan, MexicoLaboratorio de Anatomía Patológica (ANAPAT), Av. Yucatán 630, Fracc. Jardines de Mérida, Mérida 97135, Yucatan, MexicoDepartamento Recursos del Mar, Centro de Investigación y de Estudios Avanzados del IPN-Unidad Mérida, Antigua Carretera a Progreso Km. 6, Merida 97310, Yucatan, MexicoDepartamento Recursos del Mar, Centro de Investigación y de Estudios Avanzados del IPN-Unidad Mérida, Antigua Carretera a Progreso Km. 6, Merida 97310, Yucatan, MexicoFacultad de Ingeniería Química, Universidad Autónoma de Yucatán, Calle 43 No. 613 x 90, Col. Inalámbrica, Mérida 97069, Yucatan, MexicoDepartamento de Fisiología y Farmacología, Facultad de Medicina, Universidad Autónoma del Estado de Morelos, Calle Leñeros s/n, Col. Los Volcanes, Cuernavaca 62350, Morelos, MexicoDepartamento Recursos del Mar, Centro de Investigación y de Estudios Avanzados del IPN-Unidad Mérida, Antigua Carretera a Progreso Km. 6, Merida 97310, Yucatan, MexicoDepartamento Recursos del Mar, Centro de Investigación y de Estudios Avanzados del IPN-Unidad Mérida, Antigua Carretera a Progreso Km. 6, Merida 97310, Yucatan, MexicoDepartamento Recursos del Mar, Centro de Investigación y de Estudios Avanzados del IPN-Unidad Mérida, Antigua Carretera a Progreso Km. 6, Merida 97310, Yucatan, MexicoIMDEA Food Institute, CEI UAM+CSIC, Carretera de Cantoblanco 8, 28049 Madrid, SpainCentro Regional de Investigación Pesquera de Ensenada, Carretera Tijuana-Ensenada Km. 107.5, El Sauzal de Rodríguez, Ensenada 22860, Baja California, MexicoDepartamento de Física Aplicada, Centro de Investigación y de Estudios Avanzados del IPN-Unidad Mérida, Antigua Carretera a Progreso Km. 6, Mérida 97310, Yucatan, MexicoInstituto Politécnico Nacional, Unidad de Investigación, Desarrollo e Innovación Médica y Biotecnológica (UDIMEB), Prolongación de Carpio y Plan de Ayala s/n, Col. Santo Tomás, Del. Miguel Hidalgo, Ciudad de México 11340, MexicoImmunology and Proteomics Laboratory, Mexico Children’s Hospital “Federico Gómez”, Mexico City 06720, MexicoCátedras CONACYT-Fundacion IMSS, A.C., CONACYT, Av. Insurgentes Sur 1582, Del. Benito Juárez, Col. Crédito Constructor, Ciudad de Mexico 03940, MexicoDepartamento Recursos del Mar, Centro de Investigación y de Estudios Avanzados del IPN-Unidad Mérida, Antigua Carretera a Progreso Km. 6, Merida 97310, Yucatan, MexicoIMDEA Food Institute, CEI UAM+CSIC, Carretera de Cantoblanco 8, 28049 Madrid, SpainSea cucumber body wall contains several naturally occurring bioactive components that possess health-promoting properties. <i>Isostichopus badionotus</i> from Yucatan, Mexico is heavily fished, but little is known about its bioactive constituents. We previously established that <i>I. badionotus</i> meal had potent anti-inflammatory properties in vivo. We have now screened some of its constituents for anti-inflammatory activity in vitro. Glycosaminoglycan and soluble protein preparations reduced 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammatory responses in HaCaT cells while an ethanol extract had a limited effect. The primary glycosaminoglycan (fucosylated chondroitin sulfate; FCS) was purified and tested for anti-inflammatory activity in vivo. FCS modulated the expression of critical genes, including NF-ĸB, TNFα, iNOS, and COX-2, and attenuated inflammation and tissue damage caused by TPA in a mouse ear inflammation model. It also mitigated colonic colitis caused in mice by dextran sodium sulfate. FCS from <i>I. badionotus</i> of the Yucatan Peninsula thus had strong anti-inflammatory properties in vivo.https://www.mdpi.com/2072-6643/12/6/1698holothuroidsglycosaminoglycansinflammationear-inflammation |
| spellingShingle | Leticia Olivera-Castillo George Grant Nuvia Kantún-Moreno Hirian A. Barrera-Pérez Jorge Montero Miguel A. Olvera-Novoa Leydi M. Carrillo-Cocom Juan J. Acevedo Cesar Puerto-Castillo Victor May Solís Juan A. Pérez-Vega Judit Gil-Zamorano Enrique Hernández-Garibay María A. Fernández-Herrera Mayra Pérez-Tapia Oscar Medina-Contreras Jairo R. Villanueva-Toledo Rossanna Rodriguez-Canul Alberto Dávalos A Glycosaminoglycan-Rich Fraction from Sea Cucumber <i>Isostichopus badionotus</i> Has Potent Anti-Inflammatory Properties In Vitro and In Vivo Nutrients holothuroids glycosaminoglycans inflammation ear-inflammation |
| title | A Glycosaminoglycan-Rich Fraction from Sea Cucumber <i>Isostichopus badionotus</i> Has Potent Anti-Inflammatory Properties In Vitro and In Vivo |
| title_full | A Glycosaminoglycan-Rich Fraction from Sea Cucumber <i>Isostichopus badionotus</i> Has Potent Anti-Inflammatory Properties In Vitro and In Vivo |
| title_fullStr | A Glycosaminoglycan-Rich Fraction from Sea Cucumber <i>Isostichopus badionotus</i> Has Potent Anti-Inflammatory Properties In Vitro and In Vivo |
| title_full_unstemmed | A Glycosaminoglycan-Rich Fraction from Sea Cucumber <i>Isostichopus badionotus</i> Has Potent Anti-Inflammatory Properties In Vitro and In Vivo |
| title_short | A Glycosaminoglycan-Rich Fraction from Sea Cucumber <i>Isostichopus badionotus</i> Has Potent Anti-Inflammatory Properties In Vitro and In Vivo |
| title_sort | glycosaminoglycan rich fraction from sea cucumber i isostichopus badionotus i has potent anti inflammatory properties in vitro and in vivo |
| topic | holothuroids glycosaminoglycans inflammation ear-inflammation |
| url | https://www.mdpi.com/2072-6643/12/6/1698 |
| work_keys_str_mv | AT leticiaoliveracastillo aglycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT georgegrant aglycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT nuviakantunmoreno aglycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT hirianabarreraperez aglycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT jorgemontero aglycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT miguelaolveranovoa aglycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT leydimcarrillococom aglycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT juanjacevedo aglycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT cesarpuertocastillo aglycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT victormaysolis aglycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT juanaperezvega aglycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT juditgilzamorano aglycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT enriquehernandezgaribay aglycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT mariaafernandezherrera aglycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT mayrapereztapia aglycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT oscarmedinacontreras aglycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT jairorvillanuevatoledo aglycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT rossannarodriguezcanul aglycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT albertodavalos aglycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT leticiaoliveracastillo glycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT georgegrant glycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT nuviakantunmoreno glycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT hirianabarreraperez glycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT jorgemontero glycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT miguelaolveranovoa glycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT leydimcarrillococom glycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT juanjacevedo glycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT cesarpuertocastillo glycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT victormaysolis glycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT juanaperezvega glycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT juditgilzamorano glycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT enriquehernandezgaribay glycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT mariaafernandezherrera glycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT mayrapereztapia glycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT oscarmedinacontreras glycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT jairorvillanuevatoledo glycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT rossannarodriguezcanul glycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo AT albertodavalos glycosaminoglycanrichfractionfromseacucumberiisostichopusbadionotusihaspotentantiinflammatorypropertiesinvitroandinvivo |