Epigallocatechin-3-gallate alleviates trans, trans-2,4-decadienal-induced endothelial pyroptosis and dysfunction by inhibiting NLRP3 inflammasome activation
Trans, trans-2,4-decadienal (tt-DDE) is a highly reactive unsaturated aldehyde that widely present in foods. This study aimed to investigate the toxic effects of tt-DDE exposure on human umbilical vein endothelial cells (HUVECs) and the possible protective effects of green tea catechins. tt-DDE expo...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-02-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623000282 |
| _version_ | 1811172047052603392 |
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| author | Yuanyuan Hu Zhengqi Liu Xiaoya Tao Jinjin Li Zuoxu Hou Xiaoming Guo Dayong Zhou Mingfu Wang Beiwei Zhu |
| author_facet | Yuanyuan Hu Zhengqi Liu Xiaoya Tao Jinjin Li Zuoxu Hou Xiaoming Guo Dayong Zhou Mingfu Wang Beiwei Zhu |
| author_sort | Yuanyuan Hu |
| collection | DOAJ |
| description | Trans, trans-2,4-decadienal (tt-DDE) is a highly reactive unsaturated aldehyde that widely present in foods. This study aimed to investigate the toxic effects of tt-DDE exposure on human umbilical vein endothelial cells (HUVECs) and the possible protective effects of green tea catechins. tt-DDE exposure induced pyroptosis and NLRP3 inflammasome activation in endothelial cells, as evidenced by increased LDH release and PI-positive cells, and elevated protein expressions of NLRP3, cleaved caspase-1, and GSDMD-N. NLRP3 inhibitor (MCC950) efficiently suppressed the tt-DDE-induced NLRP3 inflammasome activation and pyroptosis. Additionally, epigallocatechin-3-gallate (EGCG) showed the strongest activity among the four green tea catechins, which significantly alleviated tt-DDE-induced cytotoxicity. Moreover, EGCG effectively attenuated tt-DDE-induced endothelial cell pyroptosis and dysfunction by inhibiting NLRP3 inflammasome. Results of cultured mesenteric arteries further confirmed that EGCG prevented tt-DDE-induced endothelial dysfunction and pyroptosis. These results provide novel insights into tt-DDE-induced endothelial injury, and demonstrate the protective role of EGCG against tt-DDE-associated endothelial toxicity. |
| first_indexed | 2024-04-10T17:24:02Z |
| format | Article |
| id | doaj.art-ab1d61724b704087a44d9fb7e4cc7627 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-04-10T17:24:02Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-ab1d61724b704087a44d9fb7e4cc76272023-02-05T04:15:39ZengElsevierJournal of Functional Foods1756-46462023-02-01101105428Epigallocatechin-3-gallate alleviates trans, trans-2,4-decadienal-induced endothelial pyroptosis and dysfunction by inhibiting NLRP3 inflammasome activationYuanyuan Hu0Zhengqi Liu1Xiaoya Tao2Jinjin Li3Zuoxu Hou4Xiaoming Guo5Dayong Zhou6Mingfu Wang7Beiwei Zhu8College of Civil and Transportation Engineering, Shenzhen University, Shenzhen 518060, PR China; Shenzhen Key Laboratory of Food Nutrition and Health, Institute for Advanced Study and Institute for Innovative Development of Food Industry, Shenzhen University, Shenzhen 518060, PR China; School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR ChinaCollege of Civil and Transportation Engineering, Shenzhen University, Shenzhen 518060, PR China; Shenzhen Key Laboratory of Food Nutrition and Health, Institute for Advanced Study and Institute for Innovative Development of Food Industry, Shenzhen University, Shenzhen 518060, PR ChinaCollege of Civil and Transportation Engineering, Shenzhen University, Shenzhen 518060, PR China; Shenzhen Key Laboratory of Food Nutrition and Health, Institute for Advanced Study and Institute for Innovative Development of Food Industry, Shenzhen University, Shenzhen 518060, PR ChinaShenzhen Key Laboratory of Food Nutrition and Health, Institute for Advanced Study and Institute for Innovative Development of Food Industry, Shenzhen University, Shenzhen 518060, PR ChinaShenzhen Key Laboratory of Food Nutrition and Health, Institute for Advanced Study and Institute for Innovative Development of Food Industry, Shenzhen University, Shenzhen 518060, PR ChinaShenzhen Key Laboratory of Food Nutrition and Health, Institute for Advanced Study and Institute for Innovative Development of Food Industry, Shenzhen University, Shenzhen 518060, PR ChinaShenzhen Key Laboratory of Food Nutrition and Health, Institute for Advanced Study and Institute for Innovative Development of Food Industry, Shenzhen University, Shenzhen 518060, PR China; School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; Corresponding author at: Shenzhen Key Laboratory of Food Nutrition and Health, Institute for Advanced Study and Institute for Innovative Development of Food Industry, Shenzhen University, Shenzhen 518060, PR China.Shenzhen Key Laboratory of Food Nutrition and Health, Institute for Advanced Study and Institute for Innovative Development of Food Industry, Shenzhen University, Shenzhen 518060, PR ChinaCollege of Civil and Transportation Engineering, Shenzhen University, Shenzhen 518060, PR China; Shenzhen Key Laboratory of Food Nutrition and Health, Institute for Advanced Study and Institute for Innovative Development of Food Industry, Shenzhen University, Shenzhen 518060, PR China; School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR ChinaTrans, trans-2,4-decadienal (tt-DDE) is a highly reactive unsaturated aldehyde that widely present in foods. This study aimed to investigate the toxic effects of tt-DDE exposure on human umbilical vein endothelial cells (HUVECs) and the possible protective effects of green tea catechins. tt-DDE exposure induced pyroptosis and NLRP3 inflammasome activation in endothelial cells, as evidenced by increased LDH release and PI-positive cells, and elevated protein expressions of NLRP3, cleaved caspase-1, and GSDMD-N. NLRP3 inhibitor (MCC950) efficiently suppressed the tt-DDE-induced NLRP3 inflammasome activation and pyroptosis. Additionally, epigallocatechin-3-gallate (EGCG) showed the strongest activity among the four green tea catechins, which significantly alleviated tt-DDE-induced cytotoxicity. Moreover, EGCG effectively attenuated tt-DDE-induced endothelial cell pyroptosis and dysfunction by inhibiting NLRP3 inflammasome. Results of cultured mesenteric arteries further confirmed that EGCG prevented tt-DDE-induced endothelial dysfunction and pyroptosis. These results provide novel insights into tt-DDE-induced endothelial injury, and demonstrate the protective role of EGCG against tt-DDE-associated endothelial toxicity.http://www.sciencedirect.com/science/article/pii/S1756464623000282Epigallocatechin-3-gallateVascular protectiveTrans, trans-2,4-decadienalPyroptosisNLRP3 inflammasomeEndothelial cell |
| spellingShingle | Yuanyuan Hu Zhengqi Liu Xiaoya Tao Jinjin Li Zuoxu Hou Xiaoming Guo Dayong Zhou Mingfu Wang Beiwei Zhu Epigallocatechin-3-gallate alleviates trans, trans-2,4-decadienal-induced endothelial pyroptosis and dysfunction by inhibiting NLRP3 inflammasome activation Journal of Functional Foods Epigallocatechin-3-gallate Vascular protective Trans, trans-2,4-decadienal Pyroptosis NLRP3 inflammasome Endothelial cell |
| title | Epigallocatechin-3-gallate alleviates trans, trans-2,4-decadienal-induced endothelial pyroptosis and dysfunction by inhibiting NLRP3 inflammasome activation |
| title_full | Epigallocatechin-3-gallate alleviates trans, trans-2,4-decadienal-induced endothelial pyroptosis and dysfunction by inhibiting NLRP3 inflammasome activation |
| title_fullStr | Epigallocatechin-3-gallate alleviates trans, trans-2,4-decadienal-induced endothelial pyroptosis and dysfunction by inhibiting NLRP3 inflammasome activation |
| title_full_unstemmed | Epigallocatechin-3-gallate alleviates trans, trans-2,4-decadienal-induced endothelial pyroptosis and dysfunction by inhibiting NLRP3 inflammasome activation |
| title_short | Epigallocatechin-3-gallate alleviates trans, trans-2,4-decadienal-induced endothelial pyroptosis and dysfunction by inhibiting NLRP3 inflammasome activation |
| title_sort | epigallocatechin 3 gallate alleviates trans trans 2 4 decadienal induced endothelial pyroptosis and dysfunction by inhibiting nlrp3 inflammasome activation |
| topic | Epigallocatechin-3-gallate Vascular protective Trans, trans-2,4-decadienal Pyroptosis NLRP3 inflammasome Endothelial cell |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623000282 |
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