Systematic Quantification of GFP-tagged Protein Foci in Schizosaccharomyces pombe Nuclei

DNA damage repair proteins form foci in response to DNA damaging agents. The efficiency and integrity of the DNA repair pathway of a particular eukaryotic (mutant) strain is usually determined by the number of foci formed compared with their wild-type counterpart. Conventionally, focus number is det...

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Bibliographic Details
Main Authors: Kim Lim, Ee Chen
Format: Article
Language:English
Published: Bio-protocol LLC 2018-12-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=3117&type=0