A novel twin‐column continuous chromatography approach for separation and enrichment of monoclonal antibody charge variants

A novel twin‐column continuous chromatography approach for separation and enrichment of monoclonal antibody charge variants

Abstract Downstream processing of mAb charge variants is difficult owing to their similar molecular structures and surface charge properties. This study aimed to apply a novel twin‐column continuous chromatography (called N‐rich mode) to separate and enrich acidic variants of an IgG1 mAb. Besides, a...

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Bibliographic Details
Main Authors: Shu‐Ying Jing, Ce Shi, Hui Yi Leong, Jun‐Jie Yuan, Dong Gao, Hai‐Bin Wang, Shan‐Jing Yao, Dong‐Qiang Lin
Format: Article
Language:English
Published: Wiley-VCH 2021-06-01
Series:Engineering in Life Sciences
Subjects:
charge variant
continuous chromatography
enrichment
monoclonal antibody
separation
Online Access:https://doi.org/10.1002/elsc.202000094

Internet

https://doi.org/10.1002/elsc.202000094

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