Enzyme-assisted hydrolyzation, chemical characterization, and lipid-lowering activity of crude sulfated polysaccharides from Sargassum coreanum
Brown seaweed, which is an abundant source of secondary metabolites, contains high levels of sulfated polysaccharides and possesses various biological properties. Here, we aimed to determine the chemical characteristics and evaluate the potential lipid-lowering effects of sulfated polysaccharides fr...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S175646462300227X |
| _version_ | 1797749192967323648 |
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| author | Hyo-Geun Lee Jimin Hyun H.H.A.C.K. Jayawardhana N.M. Liyanage D.P. Nagahawatta Min-Cheol Kang You-Jin Jeon |
| author_facet | Hyo-Geun Lee Jimin Hyun H.H.A.C.K. Jayawardhana N.M. Liyanage D.P. Nagahawatta Min-Cheol Kang You-Jin Jeon |
| author_sort | Hyo-Geun Lee |
| collection | DOAJ |
| description | Brown seaweed, which is an abundant source of secondary metabolites, contains high levels of sulfated polysaccharides and possesses various biological properties. Here, we aimed to determine the chemical characteristics and evaluate the potential lipid-lowering effects of sulfated polysaccharides from Sargassum coreanum (SPSC) on differentiated 3T3-L1 cells. Treatment of differentiated 3T3-L1 cells with MCS markedly reduced the intracellular lipid accumulation by over 50% at 200 μg/mL of MCS. In addition, the key adipogenic peroxisome proliferator-activated receptor γ, CCAAT/enhancer-binding protein α, lipogenic sterol regulatory element-binding protein 1, lipolytic adipose triglyceride lipase, and AMP-activated protein kinase were identified in 3T3-L1 cells. In the in vivo study, alamarBlue staining showed that energy expenditure was significantly (* p < 0.05, *** p < 0.01) increased in MCS-treated zebrafish embryos. These findings suggested that the crude polysaccharides from Sargassum coreanum possesses intracellular lipid-inhibitory activity and could be utilized as a candidate for lipid-lowering functional food products. |
| first_indexed | 2024-03-12T16:15:45Z |
| format | Article |
| id | doaj.art-ab3df3de3e524e558ae807fc3e3e82ca |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-03-12T16:15:45Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-ab3df3de3e524e558ae807fc3e3e82ca2023-08-09T04:32:22ZengElsevierJournal of Functional Foods1756-46462023-08-01107105627Enzyme-assisted hydrolyzation, chemical characterization, and lipid-lowering activity of crude sulfated polysaccharides from Sargassum coreanumHyo-Geun Lee0Jimin Hyun1H.H.A.C.K. Jayawardhana2N.M. Liyanage3D.P. Nagahawatta4Min-Cheol Kang5You-Jin Jeon6Department of Marine Life Sciences, Jeju National University, Jeju, 63243, Republic of KoreaDepartment of Marine Life Sciences, Jeju National University, Jeju, 63243, Republic of KoreaDepartment of Marine Life Sciences, Jeju National University, Jeju, 63243, Republic of KoreaDepartment of Marine Life Sciences, Jeju National University, Jeju, 63243, Republic of KoreaDepartment of Marine Life Sciences, Jeju National University, Jeju, 63243, Republic of KoreaResearch Group of Process Engineering, Korea Food Research Institute, Wanju, 55365, Republic of Korea; Corresponding author.Department of Marine Life Sciences, Jeju National University, Jeju, 63243, Republic of Korea; Corresponding author.Brown seaweed, which is an abundant source of secondary metabolites, contains high levels of sulfated polysaccharides and possesses various biological properties. Here, we aimed to determine the chemical characteristics and evaluate the potential lipid-lowering effects of sulfated polysaccharides from Sargassum coreanum (SPSC) on differentiated 3T3-L1 cells. Treatment of differentiated 3T3-L1 cells with MCS markedly reduced the intracellular lipid accumulation by over 50% at 200 μg/mL of MCS. In addition, the key adipogenic peroxisome proliferator-activated receptor γ, CCAAT/enhancer-binding protein α, lipogenic sterol regulatory element-binding protein 1, lipolytic adipose triglyceride lipase, and AMP-activated protein kinase were identified in 3T3-L1 cells. In the in vivo study, alamarBlue staining showed that energy expenditure was significantly (* p < 0.05, *** p < 0.01) increased in MCS-treated zebrafish embryos. These findings suggested that the crude polysaccharides from Sargassum coreanum possesses intracellular lipid-inhibitory activity and could be utilized as a candidate for lipid-lowering functional food products.http://www.sciencedirect.com/science/article/pii/S175646462300227XLipid lowering activitySargassum coreanumSulfated polysaccharidesLipogenesisAdipogenesis3T3-L1 |
| spellingShingle | Hyo-Geun Lee Jimin Hyun H.H.A.C.K. Jayawardhana N.M. Liyanage D.P. Nagahawatta Min-Cheol Kang You-Jin Jeon Enzyme-assisted hydrolyzation, chemical characterization, and lipid-lowering activity of crude sulfated polysaccharides from Sargassum coreanum Journal of Functional Foods Lipid lowering activity Sargassum coreanum Sulfated polysaccharides Lipogenesis Adipogenesis 3T3-L1 |
| title | Enzyme-assisted hydrolyzation, chemical characterization, and lipid-lowering activity of crude sulfated polysaccharides from Sargassum coreanum |
| title_full | Enzyme-assisted hydrolyzation, chemical characterization, and lipid-lowering activity of crude sulfated polysaccharides from Sargassum coreanum |
| title_fullStr | Enzyme-assisted hydrolyzation, chemical characterization, and lipid-lowering activity of crude sulfated polysaccharides from Sargassum coreanum |
| title_full_unstemmed | Enzyme-assisted hydrolyzation, chemical characterization, and lipid-lowering activity of crude sulfated polysaccharides from Sargassum coreanum |
| title_short | Enzyme-assisted hydrolyzation, chemical characterization, and lipid-lowering activity of crude sulfated polysaccharides from Sargassum coreanum |
| title_sort | enzyme assisted hydrolyzation chemical characterization and lipid lowering activity of crude sulfated polysaccharides from sargassum coreanum |
| topic | Lipid lowering activity Sargassum coreanum Sulfated polysaccharides Lipogenesis Adipogenesis 3T3-L1 |
| url | http://www.sciencedirect.com/science/article/pii/S175646462300227X |
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