Unmarked Gene Editing in Clavibacter michiganensis Using CRISPR/Cas9 and 5-Fluorocytosine Counterselection

Unmarked Gene Editing in Clavibacter michiganensis Using CRISPR/Cas9 and 5-Fluorocytosine Counterselection

Plant-pathogenic bacteria in the genus Clavibacter are important quarantine species that cause considerable economic loss worldwide. The development of effective gene editing techniques and additional selectable markers is essential to expedite gene functional analysis in this important Gram-positiv...

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Bibliographic Details
Main Authors: Xing Chen, Qing Tan, Qingyang Lyu, Chengxuan Yu, Na Jiang, Jianqiang Li, Laixin Luo
Format: Article
Language:English
Published: The American Phytopathological Society 2022-01-01
Series:Molecular Plant-Microbe Interactions
Subjects:
5-fluorocytosine
bacterial pathogenesis
Clavibacter michiganensis
CRISPR/Cas9
homologous recombination
Online Access:https://apsjournals.apsnet.org/doi/10.1094/MPMI-07-21-0179-TA

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https://apsjournals.apsnet.org/doi/10.1094/MPMI-07-21-0179-TA

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