A Self-Assembled G-Quadruplex/Hemin DNAzyme-Driven DNA Walker Strategy for Sensitive and Rapid Detection of Lead Ions Based on Rolling Circle Amplification
Herein, a sensitive biosensor is constructed based on a novel rolling circle amplification (RCA) for colorimetric quantification of lead ion (Pb<sup>2+</sup>). At the detection system, GR5 DNAzymes are modified on the surface of an immunomagnetic bead, and Pb<sup>2+</sup> is...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/8/761 |
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| author | Yuhan Wang Jiaxuan Xiao Xiaona Lin Amira Waheed Ayyanu Ravikumar Zhen Zhang Yanmin Zou Chengshui Chen |
| author_facet | Yuhan Wang Jiaxuan Xiao Xiaona Lin Amira Waheed Ayyanu Ravikumar Zhen Zhang Yanmin Zou Chengshui Chen |
| author_sort | Yuhan Wang |
| collection | DOAJ |
| description | Herein, a sensitive biosensor is constructed based on a novel rolling circle amplification (RCA) for colorimetric quantification of lead ion (Pb<sup>2+</sup>). At the detection system, GR5 DNAzymes are modified on the surface of an immunomagnetic bead, and Pb<sup>2+</sup> is captured by the aptamer, inducing the disintegration of the GR5 DNAzyme and the release of the DNA walker. After the introduction of the template DNA, T4 DNA ligase, and phi29 DNA polymerase, an RCA is initiated for the sensitivity improvement of this method. Moreover, a G4-hemin DNAzyme is formed as a colorimetric signal, owing to its peroxide-like activity to catalyze the TMB-H<sub>2</sub>O<sub>2</sub> substrate. Under the optimized conditions, the limit of detection (LOD) of this fabricated biosensor could reach 3.3 pM for Pb<sup>2+</sup> with a concentration in the range of 0.01–1000 nM. Furthermore, the results of real samples analysis demonstrate its satisfactory accuracy, implying its great potential in the rapid detection of heavy metals in the environment. |
| first_indexed | 2024-03-11T00:05:40Z |
| format | Article |
| id | doaj.art-ab58b9d2279043498ed85c8282057a66 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-11T00:05:40Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-ab58b9d2279043498ed85c8282057a662023-11-19T00:24:47ZengMDPI AGBiosensors2079-63742023-07-0113876110.3390/bios13080761A Self-Assembled G-Quadruplex/Hemin DNAzyme-Driven DNA Walker Strategy for Sensitive and Rapid Detection of Lead Ions Based on Rolling Circle AmplificationYuhan Wang0Jiaxuan Xiao1Xiaona Lin2Amira Waheed3Ayyanu Ravikumar4Zhen Zhang5Yanmin Zou6Chengshui Chen7School of Emergency Management, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Emergency Management, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, ChinaDepartment of Pulmonary and Critical Care Medicine, The Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People’s Hospital, Quzhou 324000, ChinaDepartment of Pulmonary and Critical Care Medicine, The Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People’s Hospital, Quzhou 324000, ChinaSchool of Emergency Management, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Emergency Management, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Emergency Management, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, ChinaDepartment of Pulmonary and Critical Care Medicine, The Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People’s Hospital, Quzhou 324000, ChinaHerein, a sensitive biosensor is constructed based on a novel rolling circle amplification (RCA) for colorimetric quantification of lead ion (Pb<sup>2+</sup>). At the detection system, GR5 DNAzymes are modified on the surface of an immunomagnetic bead, and Pb<sup>2+</sup> is captured by the aptamer, inducing the disintegration of the GR5 DNAzyme and the release of the DNA walker. After the introduction of the template DNA, T4 DNA ligase, and phi29 DNA polymerase, an RCA is initiated for the sensitivity improvement of this method. Moreover, a G4-hemin DNAzyme is formed as a colorimetric signal, owing to its peroxide-like activity to catalyze the TMB-H<sub>2</sub>O<sub>2</sub> substrate. Under the optimized conditions, the limit of detection (LOD) of this fabricated biosensor could reach 3.3 pM for Pb<sup>2+</sup> with a concentration in the range of 0.01–1000 nM. Furthermore, the results of real samples analysis demonstrate its satisfactory accuracy, implying its great potential in the rapid detection of heavy metals in the environment.https://www.mdpi.com/2079-6374/13/8/761biosensorrapid detectionsignal amplificationrolling circle amplificationG-quadruplex |
| spellingShingle | Yuhan Wang Jiaxuan Xiao Xiaona Lin Amira Waheed Ayyanu Ravikumar Zhen Zhang Yanmin Zou Chengshui Chen A Self-Assembled G-Quadruplex/Hemin DNAzyme-Driven DNA Walker Strategy for Sensitive and Rapid Detection of Lead Ions Based on Rolling Circle Amplification Biosensors biosensor rapid detection signal amplification rolling circle amplification G-quadruplex |
| title | A Self-Assembled G-Quadruplex/Hemin DNAzyme-Driven DNA Walker Strategy for Sensitive and Rapid Detection of Lead Ions Based on Rolling Circle Amplification |
| title_full | A Self-Assembled G-Quadruplex/Hemin DNAzyme-Driven DNA Walker Strategy for Sensitive and Rapid Detection of Lead Ions Based on Rolling Circle Amplification |
| title_fullStr | A Self-Assembled G-Quadruplex/Hemin DNAzyme-Driven DNA Walker Strategy for Sensitive and Rapid Detection of Lead Ions Based on Rolling Circle Amplification |
| title_full_unstemmed | A Self-Assembled G-Quadruplex/Hemin DNAzyme-Driven DNA Walker Strategy for Sensitive and Rapid Detection of Lead Ions Based on Rolling Circle Amplification |
| title_short | A Self-Assembled G-Quadruplex/Hemin DNAzyme-Driven DNA Walker Strategy for Sensitive and Rapid Detection of Lead Ions Based on Rolling Circle Amplification |
| title_sort | self assembled g quadruplex hemin dnazyme driven dna walker strategy for sensitive and rapid detection of lead ions based on rolling circle amplification |
| topic | biosensor rapid detection signal amplification rolling circle amplification G-quadruplex |
| url | https://www.mdpi.com/2079-6374/13/8/761 |
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