High-Performance Subtractive Hybridization of cDNAs by Covalent Bonding Between Specific Complementary Nucleotides
We have developed an improved subtractive hybridization method that provides a fast, simple and reliable isolation of desired different sequences from two compared DNA libraries, one of which contains all unwanted homologues (subtracter) and another contains certain desired heterologues (tester). Th...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1999-05-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/99265rr06 |