Constitutive and conditional gene knockout mice for the study of intervertebral disc degeneration: Current status, decision considerations, and future possibilities
Abstract There have been an increasing number of patients with degenerative disc diseases due to the aging population. In light of this, studies on the pathogenesis of intervertebral disc degeneration have become a hot topic, and gene knockout mice have become a valuable tool in this field of resear...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-03-01
|
| Series: | JOR Spine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/jsp2.1242 |
| _version_ | 1827979089437261824 |
|---|---|
| author | Ze‐Yu Lu Peng‐Bo Chen Qing‐Yin Xu Bo Li Sheng‐Dan Jiang Lei‐Sheng Jiang Xin‐Feng Zheng |
| author_facet | Ze‐Yu Lu Peng‐Bo Chen Qing‐Yin Xu Bo Li Sheng‐Dan Jiang Lei‐Sheng Jiang Xin‐Feng Zheng |
| author_sort | Ze‐Yu Lu |
| collection | DOAJ |
| description | Abstract There have been an increasing number of patients with degenerative disc diseases due to the aging population. In light of this, studies on the pathogenesis of intervertebral disc degeneration have become a hot topic, and gene knockout mice have become a valuable tool in this field of research. With the development of science and technology, constitutive gene knockout mice can be constructed using homologous recombination, zinc finger nuclease, transcription activator‐like effector nuclease technology and clustered regularly interspaced short palindromic repeats/Cas9 (CRISPR/Cas9) system, and conditional gene knockout mice can be constructed using the Cre/LoxP system. The gene‐edited mice using these techniques have been widely used in the studies on disc degeneration. This paper reviews the development process and principles of these technologies, functions of the edited genes in disc degeneration, advantages, and disadvantages of different methods and possible targets of the specific Cre recombinase in intervertebral discs. Recommendations for the choice of suitable gene‐edited model mice are presented. At the same time, possible technological improvements in the future are also discussed. |
| first_indexed | 2024-04-09T21:31:44Z |
| format | Article |
| id | doaj.art-abb0a562c20d455a90443bc14749e2df |
| institution | Directory Open Access Journal |
| issn | 2572-1143 |
| language | English |
| last_indexed | 2024-04-09T21:31:44Z |
| publishDate | 2023-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | JOR Spine |
| spelling | doaj.art-abb0a562c20d455a90443bc14749e2df2023-03-27T08:33:00ZengWileyJOR Spine2572-11432023-03-0161n/an/a10.1002/jsp2.1242Constitutive and conditional gene knockout mice for the study of intervertebral disc degeneration: Current status, decision considerations, and future possibilitiesZe‐Yu Lu0Peng‐Bo Chen1Qing‐Yin Xu2Bo Li3Sheng‐Dan Jiang4Lei‐Sheng Jiang5Xin‐Feng Zheng6Spine Center Xinhua Hospital, Shanghai Jiao Tong University School of Medicine Shanghai ChinaSpine Center Xinhua Hospital, Shanghai Jiao Tong University School of Medicine Shanghai ChinaSpine Center Xinhua Hospital, Shanghai Jiao Tong University School of Medicine Shanghai ChinaSpine Center Xinhua Hospital, Shanghai Jiao Tong University School of Medicine Shanghai ChinaSpine Center Xinhua Hospital, Shanghai Jiao Tong University School of Medicine Shanghai ChinaSpine Center Xinhua Hospital, Shanghai Jiao Tong University School of Medicine Shanghai ChinaSpine Center Xinhua Hospital, Shanghai Jiao Tong University School of Medicine Shanghai ChinaAbstract There have been an increasing number of patients with degenerative disc diseases due to the aging population. In light of this, studies on the pathogenesis of intervertebral disc degeneration have become a hot topic, and gene knockout mice have become a valuable tool in this field of research. With the development of science and technology, constitutive gene knockout mice can be constructed using homologous recombination, zinc finger nuclease, transcription activator‐like effector nuclease technology and clustered regularly interspaced short palindromic repeats/Cas9 (CRISPR/Cas9) system, and conditional gene knockout mice can be constructed using the Cre/LoxP system. The gene‐edited mice using these techniques have been widely used in the studies on disc degeneration. This paper reviews the development process and principles of these technologies, functions of the edited genes in disc degeneration, advantages, and disadvantages of different methods and possible targets of the specific Cre recombinase in intervertebral discs. Recommendations for the choice of suitable gene‐edited model mice are presented. At the same time, possible technological improvements in the future are also discussed.https://doi.org/10.1002/jsp2.1242constructiongene edition technologygene targetingguidelineintervertebral disc degenerationknockout |
| spellingShingle | Ze‐Yu Lu Peng‐Bo Chen Qing‐Yin Xu Bo Li Sheng‐Dan Jiang Lei‐Sheng Jiang Xin‐Feng Zheng Constitutive and conditional gene knockout mice for the study of intervertebral disc degeneration: Current status, decision considerations, and future possibilities JOR Spine construction gene edition technology gene targeting guideline intervertebral disc degeneration knockout |
| title | Constitutive and conditional gene knockout mice for the study of intervertebral disc degeneration: Current status, decision considerations, and future possibilities |
| title_full | Constitutive and conditional gene knockout mice for the study of intervertebral disc degeneration: Current status, decision considerations, and future possibilities |
| title_fullStr | Constitutive and conditional gene knockout mice for the study of intervertebral disc degeneration: Current status, decision considerations, and future possibilities |
| title_full_unstemmed | Constitutive and conditional gene knockout mice for the study of intervertebral disc degeneration: Current status, decision considerations, and future possibilities |
| title_short | Constitutive and conditional gene knockout mice for the study of intervertebral disc degeneration: Current status, decision considerations, and future possibilities |
| title_sort | constitutive and conditional gene knockout mice for the study of intervertebral disc degeneration current status decision considerations and future possibilities |
| topic | construction gene edition technology gene targeting guideline intervertebral disc degeneration knockout |
| url | https://doi.org/10.1002/jsp2.1242 |
| work_keys_str_mv | AT zeyulu constitutiveandconditionalgeneknockoutmiceforthestudyofintervertebraldiscdegenerationcurrentstatusdecisionconsiderationsandfuturepossibilities AT pengbochen constitutiveandconditionalgeneknockoutmiceforthestudyofintervertebraldiscdegenerationcurrentstatusdecisionconsiderationsandfuturepossibilities AT qingyinxu constitutiveandconditionalgeneknockoutmiceforthestudyofintervertebraldiscdegenerationcurrentstatusdecisionconsiderationsandfuturepossibilities AT boli constitutiveandconditionalgeneknockoutmiceforthestudyofintervertebraldiscdegenerationcurrentstatusdecisionconsiderationsandfuturepossibilities AT shengdanjiang constitutiveandconditionalgeneknockoutmiceforthestudyofintervertebraldiscdegenerationcurrentstatusdecisionconsiderationsandfuturepossibilities AT leishengjiang constitutiveandconditionalgeneknockoutmiceforthestudyofintervertebraldiscdegenerationcurrentstatusdecisionconsiderationsandfuturepossibilities AT xinfengzheng constitutiveandconditionalgeneknockoutmiceforthestudyofintervertebraldiscdegenerationcurrentstatusdecisionconsiderationsandfuturepossibilities |