Isobutanol production by combined in vivo and in vitro metabolic engineering

Isobutanol production by combined in vivo and in vitro metabolic engineering

The production of the biofuel, isobutanol, in E. coli faces limitations due to alcohol toxicity, product inhibition, product recovery, and long-term industrial feasibility. Here we demonstrate an approach of combining both in vivo with in vitro metabolic engineering to produce isobutanol. The in viv...

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Bibliographic Details
Main Authors: Mamta Gupta, Matthew Wong, Kamran Jawed, Kamil Gedeon, Hannah Barrett, Marcelo Bassalo, Clifford Morrison, Danish Eqbal, Syed Shams Yazdani, Ryan T. Gill, Jiaqi Huang, Marc Douaisi, Jonathan Dordick, Georges Belfort, Mattheos A.G. Koffas
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:Metabolic Engineering Communications
Subjects:
α-Ketoisovalerate
Isobutanol
CRISPR
CRISPRi
Bicistronic
Fed-batch
Online Access:http://www.sciencedirect.com/science/article/pii/S2214030122000190

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http://www.sciencedirect.com/science/article/pii/S2214030122000190

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