Cell-Free Protein Synthesis by Diversifying Bacterial Transcription Machinery

Cell-Free Protein Synthesis by Diversifying Bacterial Transcription Machinery

We have evaluated several approaches to increase protein synthesis in a cell-free coupled bacterial transcription and translation system. A strong p<i>argC</i> promoter, originally isolated from a moderate thermophilic bacterium <i>Geobacillus stearothermophilus</i>, was used...

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Bibliographic Details
Main Authors: Marina Snapyan, Sylvain Robin, Garabet Yeretssian, Michèle Lecocq, Frédéric Marc, Vehary Sakanyan
Format: Article
Language:English
Published: MDPI AG 2021-10-01
Series:BioTech
Subjects:
cell-free protein synthesis
<i>rpo</i> genes coexpression
RNA polymerase
bacterial promoter
RNase I
protein overexpression
Online Access:https://www.mdpi.com/2673-6284/10/4/24

Internet

https://www.mdpi.com/2673-6284/10/4/24

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