Protocol for detecting intracellular aggregations in Arabidopsis thaliana cell wall mutants using FM4-64 staining
Summary: Here, we present a step-by step protocol to visualize intracellular aggregations in Arabidopsis mutants with cell wall secretion defects using FM4-64, a lipophilic styryl dye. We describe steps for growing seedlings, staining them with FM4-64, and identifying intracellular aggregates in cel...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725000711 |