Purification and structural characterization of three novel anti-adhesive peptides against Helicobacter pylori from corn gluten meal
More than 50% of the world population is infected with Helicobacter pylori (H. pylori). Potential anti-adhesive peptides from corn protein hydrolysate were developed using in vitro methodologies to prevent Helicobacter pylori infection. To obtain anti-adhesive peptides against Helicobacter pylori, c...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623005923 |
| _version_ | 1827382571512627200 |
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| author | Guanlong Li Xiaolan Liu Zhengfei Miao Xiqun Zheng |
| author_facet | Guanlong Li Xiaolan Liu Zhengfei Miao Xiqun Zheng |
| author_sort | Guanlong Li |
| collection | DOAJ |
| description | More than 50% of the world population is infected with Helicobacter pylori (H. pylori). Potential anti-adhesive peptides from corn protein hydrolysate were developed using in vitro methodologies to prevent Helicobacter pylori infection. To obtain anti-adhesive peptides against Helicobacter pylori, corn gluten meal (CGM) was hydrolyzed by neutrase, and the hydrolysate (CPHN) was purified by using Superdex peptide gel filtration, Q anion exchange and Mono Q anion exchange. Then, sequences of peptides were identified by Nano-LC-MS/MS, and the structural and functional properties of the identified peptides were characterized. Three novel peptides Pro-Tyr-Ala-Glu-Tyr (PYAEY, PY5), Ile-Ile-Pro-Gln-Cys-Ser (IIPQCS, II6) and Thr-Ile-Ile-Pro-Gln (TIIPQ, TI5) were identified, respectively. PY5 exhibited the best anti-adhesive activity among the three novel peptides, and its anti-adhesive activity was 40.75 ± 0.72% at 4 mg/mL. Meanwhile, PY5 also possessed significant 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity and excellent 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging capacity, with IC50 values of 1.650 ± 0.184 and 0.007 ± 0.001 mg/mL, respectively, suggesting that PY5 could alleviate stomach damage induced by Helicobacter pylori infection. In addition, molecular docking and dot blot results showed that PY5 could also conjugate with the Helicobacter pylori adhesins, SabA and BabA, through hydrophobic interactions and hydrogen bond inhibiting Helicobacter pylori adhesion. These results indicate that three novel peptides from corn protein hydrolysate have potential anti-adhesive and antioxidant effects and could serve as functional foods or drugs to prevent Helicobacter pylori infection. |
| first_indexed | 2024-03-08T14:21:28Z |
| format | Article |
| id | doaj.art-ac7dc717e2a44097b8edb9087c2d29a4 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-03-08T14:21:28Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-ac7dc717e2a44097b8edb9087c2d29a42024-01-14T05:37:29ZengElsevierJournal of Functional Foods1756-46462024-01-01112105992Purification and structural characterization of three novel anti-adhesive peptides against Helicobacter pylori from corn gluten mealGuanlong Li0Xiaolan Liu1Zhengfei Miao2Xiqun Zheng3Heilongjiang Provincial Key Laboratory of Corn Deep Processing Theory and Techology, College of Food and Bioengineering, Qiqihar University, Qiqihar 161006, PR ChinaHeilongjiang Provincial Key Laboratory of Corn Deep Processing Theory and Techology, College of Food and Bioengineering, Qiqihar University, Qiqihar 161006, PR China; Corresponding authors.Heilongjiang Provincial Key Laboratory of Corn Deep Processing Theory and Techology, College of Food and Bioengineering, Qiqihar University, Qiqihar 161006, PR ChinaCollege of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, PR China; Corresponding authors.More than 50% of the world population is infected with Helicobacter pylori (H. pylori). Potential anti-adhesive peptides from corn protein hydrolysate were developed using in vitro methodologies to prevent Helicobacter pylori infection. To obtain anti-adhesive peptides against Helicobacter pylori, corn gluten meal (CGM) was hydrolyzed by neutrase, and the hydrolysate (CPHN) was purified by using Superdex peptide gel filtration, Q anion exchange and Mono Q anion exchange. Then, sequences of peptides were identified by Nano-LC-MS/MS, and the structural and functional properties of the identified peptides were characterized. Three novel peptides Pro-Tyr-Ala-Glu-Tyr (PYAEY, PY5), Ile-Ile-Pro-Gln-Cys-Ser (IIPQCS, II6) and Thr-Ile-Ile-Pro-Gln (TIIPQ, TI5) were identified, respectively. PY5 exhibited the best anti-adhesive activity among the three novel peptides, and its anti-adhesive activity was 40.75 ± 0.72% at 4 mg/mL. Meanwhile, PY5 also possessed significant 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity and excellent 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging capacity, with IC50 values of 1.650 ± 0.184 and 0.007 ± 0.001 mg/mL, respectively, suggesting that PY5 could alleviate stomach damage induced by Helicobacter pylori infection. In addition, molecular docking and dot blot results showed that PY5 could also conjugate with the Helicobacter pylori adhesins, SabA and BabA, through hydrophobic interactions and hydrogen bond inhibiting Helicobacter pylori adhesion. These results indicate that three novel peptides from corn protein hydrolysate have potential anti-adhesive and antioxidant effects and could serve as functional foods or drugs to prevent Helicobacter pylori infection.http://www.sciencedirect.com/science/article/pii/S1756464623005923PurificationAnti-adhesive peptidesHelicobacter pyloriAntioxidant activityStructural characterization |
| spellingShingle | Guanlong Li Xiaolan Liu Zhengfei Miao Xiqun Zheng Purification and structural characterization of three novel anti-adhesive peptides against Helicobacter pylori from corn gluten meal Journal of Functional Foods Purification Anti-adhesive peptides Helicobacter pylori Antioxidant activity Structural characterization |
| title | Purification and structural characterization of three novel anti-adhesive peptides against Helicobacter pylori from corn gluten meal |
| title_full | Purification and structural characterization of three novel anti-adhesive peptides against Helicobacter pylori from corn gluten meal |
| title_fullStr | Purification and structural characterization of three novel anti-adhesive peptides against Helicobacter pylori from corn gluten meal |
| title_full_unstemmed | Purification and structural characterization of three novel anti-adhesive peptides against Helicobacter pylori from corn gluten meal |
| title_short | Purification and structural characterization of three novel anti-adhesive peptides against Helicobacter pylori from corn gluten meal |
| title_sort | purification and structural characterization of three novel anti adhesive peptides against helicobacter pylori from corn gluten meal |
| topic | Purification Anti-adhesive peptides Helicobacter pylori Antioxidant activity Structural characterization |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623005923 |
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