Novel synthetic ceramide derivatives increase intracellular calcium levels and promote epidermal keratinocyte differentiation
Ceramide is an important constituent of stratum corneum lipids, which act as both physical barriers and signal modulators. We synthesized several ceramide derivatives and investigated their effects on keratinocyte differentiation. RT-PCR and Western blotting showed that the novel synthetic ceramide...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2007-09-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520424812 |
| _version_ | 1830487013845893120 |
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| author | Yoo Bin Kwon Chang Deok Kim Jong-Kyung Youm Hyung Sub Gwak Byeong Deog Park Seung Hun Lee Saewha Jeon Bo Joong Kim Young-Joon Seo Jang-Kyu Park Jeung-Hoon Lee |
| author_facet | Yoo Bin Kwon Chang Deok Kim Jong-Kyung Youm Hyung Sub Gwak Byeong Deog Park Seung Hun Lee Saewha Jeon Bo Joong Kim Young-Joon Seo Jang-Kyu Park Jeung-Hoon Lee |
| author_sort | Yoo Bin Kwon |
| collection | DOAJ |
| description | Ceramide is an important constituent of stratum corneum lipids, which act as both physical barriers and signal modulators. We synthesized several ceramide derivatives and investigated their effects on keratinocyte differentiation. RT-PCR and Western blotting showed that the novel synthetic ceramide derivatives K6PC-4 (N-ethanol-2-hexyl-3-hydroxy-decanamide), K6PC-5, (N-ethanol-3-oxo-2-tetradecyl/hexadecyl-octadecanamide/eicosanamide)and K6PC-9 [N-(1,3-dihydroxypropyl)-2-hexyl-3-oxo-decanamide] markedly increased keratin 1 and involucrin expression in normal human epidermal keratinocytes cultured in vitro. These ceramide derivatives elicited a rapid transient increase in intracellular calcium levels, which were measured using laser scanning confocal microscopy. In addition, K6PC-4, K6PC-5, and K6PC-9 stimulated the phosphorylation of p42/44 extracellular signal-regulated kinase and c-Jun N-terminal kinase. In a reconstituted epidermis model, K6PC-4, K6PC-5, and K6PC-9 significantly increased keratin 1 expression in the suprabasal layer. These results indicate that these novel synthetic ceramide derivatives have the potential to promote keratinocyte differentiation, suggesting that the lipid molecules are applicable for treating skin diseases involving abnormal keratinocyte differentiation. |
| first_indexed | 2024-12-21T18:53:19Z |
| format | Article |
| id | doaj.art-ac8c65715e704e2cb0e8ca610906068d |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-21T18:53:19Z |
| publishDate | 2007-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-ac8c65715e704e2cb0e8ca610906068d2022-12-21T18:53:41ZengElsevierJournal of Lipid Research0022-22752007-09-0148919361943Novel synthetic ceramide derivatives increase intracellular calcium levels and promote epidermal keratinocyte differentiationYoo Bin Kwon0Chang Deok Kim1Jong-Kyung Youm2Hyung Sub Gwak3Byeong Deog Park4Seung Hun Lee5Saewha Jeon6Bo Joong Kim7Young-Joon Seo8Jang-Kyu Park9Jeung-Hoon Lee10Department of Dermatology and Research Institute for Medical Sciences, School of Medicine, Chungnam National University, Daejeon, KoreaDepartment of Dermatology and Research Institute for Medical Sciences, School of Medicine, Chungnam National University, Daejeon, KoreaBrain Korea 21 Project for Medical Science, Yonsei University, Seoul, Korea; Neopharm Co., Ltd., Daejeon, KoreaNeopharm Co., Ltd., Daejeon, KoreaNeopharm Co., Ltd., Daejeon, KoreaBrain Korea 21 Project for Medical Science, Yonsei University, Seoul, Korea; Department of Dermatology, Yonsei University College of Medicine, Seoul, KoreaTEGO Science, Seoul, KoreaDepartment of Dermatology and Research Institute for Medical Sciences, School of Medicine, Chungnam National University, Daejeon, KoreaDepartment of Dermatology and Research Institute for Medical Sciences, School of Medicine, Chungnam National University, Daejeon, KoreaDepartment of Dermatology and Research Institute for Medical Sciences, School of Medicine, Chungnam National University, Daejeon, KoreaDepartment of Dermatology and Research Institute for Medical Sciences, School of Medicine, Chungnam National University, Daejeon, KoreaCeramide is an important constituent of stratum corneum lipids, which act as both physical barriers and signal modulators. We synthesized several ceramide derivatives and investigated their effects on keratinocyte differentiation. RT-PCR and Western blotting showed that the novel synthetic ceramide derivatives K6PC-4 (N-ethanol-2-hexyl-3-hydroxy-decanamide), K6PC-5, (N-ethanol-3-oxo-2-tetradecyl/hexadecyl-octadecanamide/eicosanamide)and K6PC-9 [N-(1,3-dihydroxypropyl)-2-hexyl-3-oxo-decanamide] markedly increased keratin 1 and involucrin expression in normal human epidermal keratinocytes cultured in vitro. These ceramide derivatives elicited a rapid transient increase in intracellular calcium levels, which were measured using laser scanning confocal microscopy. In addition, K6PC-4, K6PC-5, and K6PC-9 stimulated the phosphorylation of p42/44 extracellular signal-regulated kinase and c-Jun N-terminal kinase. In a reconstituted epidermis model, K6PC-4, K6PC-5, and K6PC-9 significantly increased keratin 1 expression in the suprabasal layer. These results indicate that these novel synthetic ceramide derivatives have the potential to promote keratinocyte differentiation, suggesting that the lipid molecules are applicable for treating skin diseases involving abnormal keratinocyte differentiation.http://www.sciencedirect.com/science/article/pii/S0022227520424812K6PC-4K6PC-5K6PC-9intracellular calcium levelmitogen-activated protein kinasekeratin 1 |
| spellingShingle | Yoo Bin Kwon Chang Deok Kim Jong-Kyung Youm Hyung Sub Gwak Byeong Deog Park Seung Hun Lee Saewha Jeon Bo Joong Kim Young-Joon Seo Jang-Kyu Park Jeung-Hoon Lee Novel synthetic ceramide derivatives increase intracellular calcium levels and promote epidermal keratinocyte differentiation Journal of Lipid Research K6PC-4 K6PC-5 K6PC-9 intracellular calcium level mitogen-activated protein kinase keratin 1 |
| title | Novel synthetic ceramide derivatives increase intracellular calcium levels and promote epidermal keratinocyte differentiation |
| title_full | Novel synthetic ceramide derivatives increase intracellular calcium levels and promote epidermal keratinocyte differentiation |
| title_fullStr | Novel synthetic ceramide derivatives increase intracellular calcium levels and promote epidermal keratinocyte differentiation |
| title_full_unstemmed | Novel synthetic ceramide derivatives increase intracellular calcium levels and promote epidermal keratinocyte differentiation |
| title_short | Novel synthetic ceramide derivatives increase intracellular calcium levels and promote epidermal keratinocyte differentiation |
| title_sort | novel synthetic ceramide derivatives increase intracellular calcium levels and promote epidermal keratinocyte differentiation |
| topic | K6PC-4 K6PC-5 K6PC-9 intracellular calcium level mitogen-activated protein kinase keratin 1 |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520424812 |
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