Quality control recommendations for RNASeq using FFPE samples based on pre-sequencing lab metrics and post-sequencing bioinformatics metrics

Quality control recommendations for RNASeq using FFPE samples based on pre-sequencing lab metrics and post-sequencing bioinformatics metrics

Abstract Background Formalin-fixed, paraffin-embedded (FFPE) tissues have many advantages for identification of risk biomarkers, including wide availability and potential for extended follow-up endpoints. However, RNA derived from archival FFPE samples has limited quality. Here we identified paramet...

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Bibliographic Details
Main Authors: Yuanhang Liu, Aditya Bhagwate, Stacey J. Winham, Melissa T. Stephens, Brent W. Harker, Samantha J. McDonough, Melody L. Stallings-Mann, Ethan P. Heinzen, Robert A. Vierkant, Tanya L. Hoskin, Marlene H. Frost, Jodi M. Carter, Michael E. Pfrender, Laurie Littlepage, Derek C. Radisky, Julie M. Cunningham, Amy C. Degnim, Chen Wang
Format: Article
Language:English
Published: BMC 2022-09-01
Series:BMC Medical Genomics
Subjects:
FFPE
RNA-seq
Quality control
Breast tissue
RNA concentration
Library concentration
Online Access:https://doi.org/10.1186/s12920-022-01355-0

Internet

https://doi.org/10.1186/s12920-022-01355-0

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