Polysiloxane/PVA-glutaraldehyde hybrid composite as solid phase for immunodetections by ELISA
We developed an efficient method to prepare a hybrid inorganic-organic composite based on polyvinyl alcohol (PVA) and polysiloxane using the sol-gel disc technique. Antigen obtained from Yersinia pestis was covalently immobilized onto these discs with glutaraldehyde and used as solid phase in ELISA...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Associação Brasileira de Divulgação Científica
2002-04-01
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Series: | Brazilian Journal of Medical and Biological Research |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400008 |