Polysiloxane/PVA-glutaraldehyde hybrid composite as solid phase for immunodetections by ELISA

We developed an efficient method to prepare a hybrid inorganic-organic composite based on polyvinyl alcohol (PVA) and polysiloxane using the sol-gel disc technique. Antigen obtained from Yersinia pestis was covalently immobilized onto these discs with glutaraldehyde and used as solid phase in ELISA...

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Bibliographic Details
Main Authors: A.E. Lima Barros, A.M.P. Almeida, L.B. Carvalho Jr., W.M. Azevedo
Format: Article
Language:English
Published: Associação Brasileira de Divulgação Científica 2002-04-01
Series:Brazilian Journal of Medical and Biological Research
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400008