Polysiloxane/PVA-glutaraldehyde hybrid composite as solid phase for immunodetections by ELISA
We developed an efficient method to prepare a hybrid inorganic-organic composite based on polyvinyl alcohol (PVA) and polysiloxane using the sol-gel disc technique. Antigen obtained from Yersinia pestis was covalently immobilized onto these discs with glutaraldehyde and used as solid phase in ELISA...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2002-04-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400008 |
| _version_ | 1818180262794625024 |
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| author | A.E. Lima Barros A.M.P. Almeida L.B. Carvalho Jr. W.M. Azevedo |
| author_facet | A.E. Lima Barros A.M.P. Almeida L.B. Carvalho Jr. W.M. Azevedo |
| author_sort | A.E. Lima Barros |
| collection | DOAJ |
| description | We developed an efficient method to prepare a hybrid inorganic-organic composite based on polyvinyl alcohol (PVA) and polysiloxane using the sol-gel disc technique. Antigen obtained from Yersinia pestis was covalently immobilized onto these discs with glutaraldehyde and used as solid phase in ELISA for antibody detection in serum of rabbits experimentally immunized with plague. Using 1.25 µg antigen per disc, a peroxidase conjugate dilution of 1:4,000 and a serum dilution of 1:200 were adequate for the establishment of the procedure. These values are similar to those used for PVA-glutaraldehyde discs, plasticized filter paper discs and the polyaniline-Dacron composite discs. This procedure is comparable to that which utilizes the adsorption of the antigen to conventional PVC plates, with the amount of antigen being one fourth that employed in conventional PVC plates (5 µg/well). In addition to the performance of the polysiloxane/PVA-glutaraldehyde disc as a matrix for immunodetection, its easy synthesis and low cost are additional advantages for commercial application. |
| first_indexed | 2024-12-11T21:16:59Z |
| format | Article |
| id | doaj.art-ad295e92e13b44adb7d844a4e7b5581f |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-12-11T21:16:59Z |
| publishDate | 2002-04-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-ad295e92e13b44adb7d844a4e7b5581f2022-12-22T00:50:33ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2002-04-0135445946310.1590/S0100-879X2002000400008Polysiloxane/PVA-glutaraldehyde hybrid composite as solid phase for immunodetections by ELISAA.E. Lima BarrosA.M.P. AlmeidaL.B. Carvalho Jr.W.M. AzevedoWe developed an efficient method to prepare a hybrid inorganic-organic composite based on polyvinyl alcohol (PVA) and polysiloxane using the sol-gel disc technique. Antigen obtained from Yersinia pestis was covalently immobilized onto these discs with glutaraldehyde and used as solid phase in ELISA for antibody detection in serum of rabbits experimentally immunized with plague. Using 1.25 µg antigen per disc, a peroxidase conjugate dilution of 1:4,000 and a serum dilution of 1:200 were adequate for the establishment of the procedure. These values are similar to those used for PVA-glutaraldehyde discs, plasticized filter paper discs and the polyaniline-Dacron composite discs. This procedure is comparable to that which utilizes the adsorption of the antigen to conventional PVC plates, with the amount of antigen being one fourth that employed in conventional PVC plates (5 µg/well). In addition to the performance of the polysiloxane/PVA-glutaraldehyde disc as a matrix for immunodetection, its easy synthesis and low cost are additional advantages for commercial application.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400008PolysiloxanePolyvinyl alcoholSolid phaseImmunodetectionYersinia pestisELISA |
| spellingShingle | A.E. Lima Barros A.M.P. Almeida L.B. Carvalho Jr. W.M. Azevedo Polysiloxane/PVA-glutaraldehyde hybrid composite as solid phase for immunodetections by ELISA Brazilian Journal of Medical and Biological Research Polysiloxane Polyvinyl alcohol Solid phase Immunodetection Yersinia pestis ELISA |
| title | Polysiloxane/PVA-glutaraldehyde hybrid composite as solid phase for immunodetections by ELISA |
| title_full | Polysiloxane/PVA-glutaraldehyde hybrid composite as solid phase for immunodetections by ELISA |
| title_fullStr | Polysiloxane/PVA-glutaraldehyde hybrid composite as solid phase for immunodetections by ELISA |
| title_full_unstemmed | Polysiloxane/PVA-glutaraldehyde hybrid composite as solid phase for immunodetections by ELISA |
| title_short | Polysiloxane/PVA-glutaraldehyde hybrid composite as solid phase for immunodetections by ELISA |
| title_sort | polysiloxane pva glutaraldehyde hybrid composite as solid phase for immunodetections by elisa |
| topic | Polysiloxane Polyvinyl alcohol Solid phase Immunodetection Yersinia pestis ELISA |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400008 |
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