HDX-guided EPR spectroscopy to interrogate membrane protein dynamics

HDX-guided EPR spectroscopy to interrogate membrane protein dynamics

Summary: Solvent accessibilities of and distances between protein residues measured by pulsed-EPR approaches provide high-resolution information on dynamic protein motions. We describe protocols for the purification and site-directed spin labeling of integral membrane proteins. In our protocol, pept...

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Bibliographic Details
Main Authors: Benjamin J. Lane, Bolin Wang, Yue Ma, Antonio N. Calabrese, Hassane El Mkami, Christos Pliotas
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Biophysics
Cell Membrane
Molecular Biology
Protein Biochemistry
Protein expression and purification
Structural Biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722004427

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http://www.sciencedirect.com/science/article/pii/S2666166722004427

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