Profiling and verifying the substrates of E3 ubiquitin ligase Rsp5 in yeast cells

Profiling and verifying the substrates of E3 ubiquitin ligase Rsp5 in yeast cells

Summary: Yeast is an essential model organism for studying protein ubiquitination pathways; however, identifying the direct substrates of E3 in the cell presents a challenge. Here, we present a protocol for using the orthogonal ubiquitin transfer (OUT) cascade to profile the substrate specificity of...

Full description

Bibliographic Details
Main Authors: Shuai Fang, Geng Chen, Yiyang Wang, Rakhee Ganti, Tatiana A. Chernova, Li Zhou, Savannah E. Jacobs, Duc Duong, Hiroaki Kiyokawa, Yury O. Chernoff, Ming Li, Natalia Shcherbik, Bo Zhao, Jun Yin
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell-based Assays
Molecular Biology
Molecular/Chemical Probes
Protein Biochemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723004562

Internet

http://www.sciencedirect.com/science/article/pii/S2666166723004562

Similar Items