Extra kinetic dimensions for label discrimination
Label discrimination is challenging in fluorescence microscopy due to broad spectra and narrow lifetime distribution. Here, the authors introduce extra kinetic dimensions by illuminating reversibly photoswitchable fluorophores with different intensities, and discriminate 20 spectrally similar fluoro...
Main Authors: | , , , , , , , , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Nature Portfolio
2022-03-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-022-29172-0 |