Effect of Pollination with Gamma Irradiated Pollen on In Vitro Regeneration of Ovule Culture in Cyclamen

The obtention of homozygous lines through in situ parthenogenesis via gamma irradiation method is a comprehensive application in vegetables. However, there are a limited number of studies on in situ parthenogenesis in ornamentals. Therefore, in situ parthenogenetic capacity of more species needs to...

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Main Authors: Mehmet Tütüncü, Yeşim Yalçın Mendi
Format: Article
Language:English
Published: Turkish Science and Technology Publishing (TURSTEP) 2022-12-01
Series:Turkish Journal of Agriculture: Food Science and Technology
Subjects:
Online Access:http://www.agrifoodscience.com/index.php/TURJAF/article/view/5409
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author Mehmet Tütüncü
Yeşim Yalçın Mendi
author_facet Mehmet Tütüncü
Yeşim Yalçın Mendi
author_sort Mehmet Tütüncü
collection DOAJ
description The obtention of homozygous lines through in situ parthenogenesis via gamma irradiation method is a comprehensive application in vegetables. However, there are a limited number of studies on in situ parthenogenesis in ornamentals. Therefore, in situ parthenogenetic capacity of more species needs to be examined. For this purpose, the effects of pollination with gamma-irradiated pollen on in vitro ovule cultured in Cyclamen persicum L. were evaluated in this study. Flower buds were collected before anthesis and irradiated at different doses 50, 100, 150, 200, 300, and 450 Gy of gamma-ray using a Co-60 source. The control group was pollinated with non-irradiated pollen. Fruits were harvested 30 days after pollination, and isolated ovule explants were cultured on four different mediums in in vitro. M0 was control group containing half-strength MS basal media; M1 media additionally has 10 g/L maltose, 1.0 g/L proline, 2.0 g/L peptone, 200.0 mg/L spermidine and 0.5 mg/L kinetin; in addition to basal medium, M2 media additionally contains 10 g/L maltose, 1.0 g/L proline, 2.0 g/L peptone, 200.0 mg/L spermidine, 0.4 mg/L gibberellic acid (GA3) and 0.4 mg/L N6-benzyl adenine (BA). M3 media additionally contains 2.0 mg/L 2,4-D and 0.8 mg/L 6-( γ, γ-dimethylallylamino) purine (2iP). Plantlets started to form 10-12 weeks after the beginning of culture. The effects of nutrient media, irradiation dose, and their interactions on plant formation were statistically significant. The lowest plantlet regeneration (0.33%) was obtained from ovule explants exposed to 50 Gy gamma rays and cultured on M3 media while the highest plantlet regeneration (2.66%) was obtained from ovule explants cultured on M1 media 30 days after pollination with non-irradiated pollen grains. According to stomatal observations, there were no statistical differences between donor plant and in vitro regenerated plantlets.
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spelling doaj.art-ad640ac5acf044c3a3837b5e61378de32023-02-15T16:16:41ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2022-12-0110122415242010.24925/turjaf.v10i12.2415-2420.54092688Effect of Pollination with Gamma Irradiated Pollen on In Vitro Regeneration of Ovule Culture in CyclamenMehmet Tütüncü0Yeşim Yalçın Mendi1Department of Horticulture, Faculty of Agriculture, Ondokuz Mayıs University 55200 SamsunDepartment of Horticulture, Faculty of Agriculture, Çukurova University,01030 AdanaThe obtention of homozygous lines through in situ parthenogenesis via gamma irradiation method is a comprehensive application in vegetables. However, there are a limited number of studies on in situ parthenogenesis in ornamentals. Therefore, in situ parthenogenetic capacity of more species needs to be examined. For this purpose, the effects of pollination with gamma-irradiated pollen on in vitro ovule cultured in Cyclamen persicum L. were evaluated in this study. Flower buds were collected before anthesis and irradiated at different doses 50, 100, 150, 200, 300, and 450 Gy of gamma-ray using a Co-60 source. The control group was pollinated with non-irradiated pollen. Fruits were harvested 30 days after pollination, and isolated ovule explants were cultured on four different mediums in in vitro. M0 was control group containing half-strength MS basal media; M1 media additionally has 10 g/L maltose, 1.0 g/L proline, 2.0 g/L peptone, 200.0 mg/L spermidine and 0.5 mg/L kinetin; in addition to basal medium, M2 media additionally contains 10 g/L maltose, 1.0 g/L proline, 2.0 g/L peptone, 200.0 mg/L spermidine, 0.4 mg/L gibberellic acid (GA3) and 0.4 mg/L N6-benzyl adenine (BA). M3 media additionally contains 2.0 mg/L 2,4-D and 0.8 mg/L 6-( γ, γ-dimethylallylamino) purine (2iP). Plantlets started to form 10-12 weeks after the beginning of culture. The effects of nutrient media, irradiation dose, and their interactions on plant formation were statistically significant. The lowest plantlet regeneration (0.33%) was obtained from ovule explants exposed to 50 Gy gamma rays and cultured on M3 media while the highest plantlet regeneration (2.66%) was obtained from ovule explants cultured on M1 media 30 days after pollination with non-irradiated pollen grains. According to stomatal observations, there were no statistical differences between donor plant and in vitro regenerated plantlets.http://www.agrifoodscience.com/index.php/TURJAF/article/view/5409gynogenesishaploidizationirradiationparthenogenesisornamental plant
spellingShingle Mehmet Tütüncü
Yeşim Yalçın Mendi
Effect of Pollination with Gamma Irradiated Pollen on In Vitro Regeneration of Ovule Culture in Cyclamen
Turkish Journal of Agriculture: Food Science and Technology
gynogenesis
haploidization
irradiation
parthenogenesis
ornamental plant
title Effect of Pollination with Gamma Irradiated Pollen on In Vitro Regeneration of Ovule Culture in Cyclamen
title_full Effect of Pollination with Gamma Irradiated Pollen on In Vitro Regeneration of Ovule Culture in Cyclamen
title_fullStr Effect of Pollination with Gamma Irradiated Pollen on In Vitro Regeneration of Ovule Culture in Cyclamen
title_full_unstemmed Effect of Pollination with Gamma Irradiated Pollen on In Vitro Regeneration of Ovule Culture in Cyclamen
title_short Effect of Pollination with Gamma Irradiated Pollen on In Vitro Regeneration of Ovule Culture in Cyclamen
title_sort effect of pollination with gamma irradiated pollen on in vitro regeneration of ovule culture in cyclamen
topic gynogenesis
haploidization
irradiation
parthenogenesis
ornamental plant
url http://www.agrifoodscience.com/index.php/TURJAF/article/view/5409
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