A CRISPRi-based genetic resource to study essential Staphylococcus aureus genes

A CRISPRi-based genetic resource to study essential Staphylococcus aureus genes

ABSTRACTWe have optimized a clustered regularly interspaced short palindromic repeat (CRISPR) interference system to facilitate gene knockdown in the Gram-positive bacterial pathogen Staphylococcus aureus. Our approach used a CRISPRi system derived from Streptococcus pyogenes, which involves the co-...

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Bibliographic Details
Main Authors: Patricia Reed, Moritz Sorg, Dominik Alwardt, Lúcia Serra, Helena Veiga, Simon Schäper, Mariana G. Pinho
Format: Article
Language:English
Published: American Society for Microbiology 2024-01-01
Series:mBio
Subjects:
Staphylococcus aureus
CRISPRi
essential genes
Online Access:https://journals.asm.org/doi/10.1128/mbio.02773-23

Internet

https://journals.asm.org/doi/10.1128/mbio.02773-23

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