Method for immobilization of living and synthetic cells for high-resolution imaging and single-particle tracking

Abstract Super-resolution imaging and single-particle tracking require cells to be immobile as any movement reduces the resolution of the measurements. Here, we present a method based on APTES-glutaraldehyde coating of glass surfaces to immobilize cells without compromising their growth. Our method...

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Bibliographic Details
Main Authors: Łukasz Syga, Dian Spakman, Christiaan M. Punter, Bert Poolman
Format: Article
Language:English
Published: Nature Portfolio 2018-09-01
Series:Scientific Reports
Subjects:
Online Access:https://doi.org/10.1038/s41598-018-32166-y