The SEM-4 Transcription Factor Is Required for Regulation of the Oxidative Stress Response in Caenorhabditis elegans
Oxidative stress causes damage to cells by creating reactive oxygen species (ROS) and the overproduction of ROS have been linked to the onset of premature aging. We previously found that a brap-2 (BRCA1 associated protein 2) mutant significantly increases the expression of phase II detoxification en...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2020-09-01
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| Series: | G3: Genes, Genomes, Genetics |
| Subjects: | |
| Online Access: | http://g3journal.org/lookup/doi/10.1534/g3.120.401316 |
| _version_ | 1818437882015121408 |
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| author | Adilya Rafikova Queenie Hu Terrance J. Kubiseski |
| author_facet | Adilya Rafikova Queenie Hu Terrance J. Kubiseski |
| author_sort | Adilya Rafikova |
| collection | DOAJ |
| description | Oxidative stress causes damage to cells by creating reactive oxygen species (ROS) and the overproduction of ROS have been linked to the onset of premature aging. We previously found that a brap-2 (BRCA1 associated protein 2) mutant significantly increases the expression of phase II detoxification enzymes in C. elegans. An RNAi suppression screen to identify transcription factors involved in the production of gst-4 mRNA in brap-2 worms identified SEM-4 as a potential candidate. Here, we show that knockdown of sem-4 suppresses the activation of gst-4 caused by the mutation in brap-2. We also demonstrate that sem-4 is required for survival upon exposure to oxidative stress and that SEM-4 is required for expression of the transcription factor SKN-1C. These findings identify a novel role for SEM-4 in ROS detoxification by regulating expression of SKN-1C and the phase II detoxification genes. |
| first_indexed | 2024-12-14T17:31:44Z |
| format | Article |
| id | doaj.art-ad88bf9de7c04e67963ccc97d377f52f |
| institution | Directory Open Access Journal |
| issn | 2160-1836 |
| language | English |
| last_indexed | 2024-12-14T17:31:44Z |
| publishDate | 2020-09-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | G3: Genes, Genomes, Genetics |
| spelling | doaj.art-ad88bf9de7c04e67963ccc97d377f52f2022-12-21T22:53:05ZengOxford University PressG3: Genes, Genomes, Genetics2160-18362020-09-011093379338510.1534/g3.120.40131641The SEM-4 Transcription Factor Is Required for Regulation of the Oxidative Stress Response in Caenorhabditis elegansAdilya RafikovaQueenie HuTerrance J. KubiseskiOxidative stress causes damage to cells by creating reactive oxygen species (ROS) and the overproduction of ROS have been linked to the onset of premature aging. We previously found that a brap-2 (BRCA1 associated protein 2) mutant significantly increases the expression of phase II detoxification enzymes in C. elegans. An RNAi suppression screen to identify transcription factors involved in the production of gst-4 mRNA in brap-2 worms identified SEM-4 as a potential candidate. Here, we show that knockdown of sem-4 suppresses the activation of gst-4 caused by the mutation in brap-2. We also demonstrate that sem-4 is required for survival upon exposure to oxidative stress and that SEM-4 is required for expression of the transcription factor SKN-1C. These findings identify a novel role for SEM-4 in ROS detoxification by regulating expression of SKN-1C and the phase II detoxification genes.http://g3journal.org/lookup/doi/10.1534/g3.120.401316caenorhabditis elegans (c. elegans)oxidative stressgeneticsgene expressiontranscription factorsem-4splat-like (sall) family of transcription factors |
| spellingShingle | Adilya Rafikova Queenie Hu Terrance J. Kubiseski The SEM-4 Transcription Factor Is Required for Regulation of the Oxidative Stress Response in Caenorhabditis elegans G3: Genes, Genomes, Genetics caenorhabditis elegans (c. elegans) oxidative stress genetics gene expression transcription factor sem-4 splat-like (sall) family of transcription factors |
| title | The SEM-4 Transcription Factor Is Required for Regulation of the Oxidative Stress Response in Caenorhabditis elegans |
| title_full | The SEM-4 Transcription Factor Is Required for Regulation of the Oxidative Stress Response in Caenorhabditis elegans |
| title_fullStr | The SEM-4 Transcription Factor Is Required for Regulation of the Oxidative Stress Response in Caenorhabditis elegans |
| title_full_unstemmed | The SEM-4 Transcription Factor Is Required for Regulation of the Oxidative Stress Response in Caenorhabditis elegans |
| title_short | The SEM-4 Transcription Factor Is Required for Regulation of the Oxidative Stress Response in Caenorhabditis elegans |
| title_sort | sem 4 transcription factor is required for regulation of the oxidative stress response in caenorhabditis elegans |
| topic | caenorhabditis elegans (c. elegans) oxidative stress genetics gene expression transcription factor sem-4 splat-like (sall) family of transcription factors |
| url | http://g3journal.org/lookup/doi/10.1534/g3.120.401316 |
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