rec-YnH enables simultaneous many-by-many detection of direct protein–protein and protein–RNA interactions

Current methods to unveil protein–protein and protein–RNA interactions often involve long, costly procedures. Here the authors present a rapid yeast 2- and 3-hybrid screening pipeline to detect interactions within protein libraries or between protein libraries and RNA fragment pools.

Bibliographic Details
Main Authors: Jae-Seong Yang, Mireia Garriga-Canut, Nele Link, Carlo Carolis, Katrina Broadbent, Violeta Beltran-Sastre, Luis Serrano, Sebastian P. Maurer
Format: Article
Language:English
Published: Nature Portfolio 2018-09-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-018-06128-x