rec-YnH enables simultaneous many-by-many detection of direct protein–protein and protein–RNA interactions
Current methods to unveil protein–protein and protein–RNA interactions often involve long, costly procedures. Here the authors present a rapid yeast 2- and 3-hybrid screening pipeline to detect interactions within protein libraries or between protein libraries and RNA fragment pools.
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2018-09-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-018-06128-x |
| _version_ | 1819039691386650624 |
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| author | Jae-Seong Yang Mireia Garriga-Canut Nele Link Carlo Carolis Katrina Broadbent Violeta Beltran-Sastre Luis Serrano Sebastian P. Maurer |
| author_facet | Jae-Seong Yang Mireia Garriga-Canut Nele Link Carlo Carolis Katrina Broadbent Violeta Beltran-Sastre Luis Serrano Sebastian P. Maurer |
| author_sort | Jae-Seong Yang |
| collection | DOAJ |
| description | Current methods to unveil protein–protein and protein–RNA interactions often involve long, costly procedures. Here the authors present a rapid yeast 2- and 3-hybrid screening pipeline to detect interactions within protein libraries or between protein libraries and RNA fragment pools. |
| first_indexed | 2024-12-21T08:57:14Z |
| format | Article |
| id | doaj.art-ad8f61c4cf2f48e2a8c5190bb415f3f9 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-21T08:57:14Z |
| publishDate | 2018-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-ad8f61c4cf2f48e2a8c5190bb415f3f92022-12-21T19:09:32ZengNature PortfolioNature Communications2041-17232018-09-019111310.1038/s41467-018-06128-xrec-YnH enables simultaneous many-by-many detection of direct protein–protein and protein–RNA interactionsJae-Seong Yang0Mireia Garriga-Canut1Nele Link2Carlo Carolis3Katrina Broadbent4Violeta Beltran-Sastre5Luis Serrano6Sebastian P. Maurer7Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology (BIST)Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology (BIST)Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology (BIST)Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology (BIST)Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology (BIST)Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology (BIST)Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology (BIST)Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology (BIST)Current methods to unveil protein–protein and protein–RNA interactions often involve long, costly procedures. Here the authors present a rapid yeast 2- and 3-hybrid screening pipeline to detect interactions within protein libraries or between protein libraries and RNA fragment pools.https://doi.org/10.1038/s41467-018-06128-x |
| spellingShingle | Jae-Seong Yang Mireia Garriga-Canut Nele Link Carlo Carolis Katrina Broadbent Violeta Beltran-Sastre Luis Serrano Sebastian P. Maurer rec-YnH enables simultaneous many-by-many detection of direct protein–protein and protein–RNA interactions Nature Communications |
| title | rec-YnH enables simultaneous many-by-many detection of direct protein–protein and protein–RNA interactions |
| title_full | rec-YnH enables simultaneous many-by-many detection of direct protein–protein and protein–RNA interactions |
| title_fullStr | rec-YnH enables simultaneous many-by-many detection of direct protein–protein and protein–RNA interactions |
| title_full_unstemmed | rec-YnH enables simultaneous many-by-many detection of direct protein–protein and protein–RNA interactions |
| title_short | rec-YnH enables simultaneous many-by-many detection of direct protein–protein and protein–RNA interactions |
| title_sort | rec ynh enables simultaneous many by many detection of direct protein protein and protein rna interactions |
| url | https://doi.org/10.1038/s41467-018-06128-x |
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