rec-YnH enables simultaneous many-by-many detection of direct protein–protein and protein–RNA interactions
Current methods to unveil protein–protein and protein–RNA interactions often involve long, costly procedures. Here the authors present a rapid yeast 2- and 3-hybrid screening pipeline to detect interactions within protein libraries or between protein libraries and RNA fragment pools.
Main Authors: | Jae-Seong Yang, Mireia Garriga-Canut, Nele Link, Carlo Carolis, Katrina Broadbent, Violeta Beltran-Sastre, Luis Serrano, Sebastian P. Maurer |
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Format: | Article |
Language: | English |
Published: |
Nature Portfolio
2018-09-01
|
Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-018-06128-x |
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