Application of CRISPR/Cas9 System for Plasmid Elimination and Bacterial Killing of Bacillus cereus Group Strains

Application of CRISPR/Cas9 System for Plasmid Elimination and Bacterial Killing of Bacillus cereus Group Strains

The CRISPR-Cas system has been widely applied in prokaryotic genome editing with its high efficiency and easy operation. We constructed some “scissors plasmids” via using the temperature-sensitive pJOE8999 shuttle plasmid, which carry the different 20nt (N20) guiding the Cas9 nuclease as a scissors...

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Bibliographic Details
Main Authors: Xiaojing Wang, Yufei Lyu, Siya Wang, Qingfang Zheng, Erling Feng, Li Zhu, Chao Pan, Shenghou Wang, Dongshu Wang, Xiankai Liu, Hengliang Wang
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-06-01
Series:Frontiers in Microbiology
Subjects:
CRISPR/Cas9
sgRNA
B. cereus group
plasmid curing
virulence plasmid
sequence-specific antimicrobials
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2021.536357/full

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https://www.frontiersin.org/articles/10.3389/fmicb.2021.536357/full

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