Identification of Cardiac CircRNAs in Mice With CVB3-Induced Myocarditis
Background: Viral myocarditis could initiate various immune response to the myocardium, resulting in myocyte damage and subsequent cardiac dysfunction. The expression profile and functions of circRNAs in this process are unknown.Methods: Fulminant myocarditis (FM) and non-FM models were induced by c...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-02-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2022.760509/full |
| _version_ | 1828851353234964480 |
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| author | Xiang Nie Jiahui Fan Huihui Li Jin Wang Rong Xie Chen Chen Dao Wen Wang |
| author_facet | Xiang Nie Jiahui Fan Huihui Li Jin Wang Rong Xie Chen Chen Dao Wen Wang |
| author_sort | Xiang Nie |
| collection | DOAJ |
| description | Background: Viral myocarditis could initiate various immune response to the myocardium, resulting in myocyte damage and subsequent cardiac dysfunction. The expression profile and functions of circRNAs in this process are unknown.Methods: Fulminant myocarditis (FM) and non-FM models were induced by coxsackie B3 virus (CVB3) infection in A/J mice and C57BL/6 mice, respectively. CircRNAs expression profile was identified by RNA-seq. Quantitative RT-PCR, Spearman rank correlation, KEGG pathway, GO analysis, Western blot and flow cytometry were performed for functional analysis.Results: Severer inflammatory cell infiltration and cardiomyocyte necrosis were presented in CVB3-treated A/J mice than those in C57BL/6 mice. The dysregulated circRNAs in both of the mouse strains displayed strong correlation with the immune response, but dysregulated circRNAs in A/J mice were more prone to cardiac dysfunction. KEGG analysis indicated that the target genes of dysregulated circRNAs in A/J mice were mainly involved in viral infection, T cell and B cell receptor signaling pathways, while the target genes of dysregulated circRNAs in C57BL/6 mice were unrelated to immune pathways. Furthermore, knockdown of circArhgap32 that was downregulated in CVB3-treated A/J mice promoted cardiomyocyte apoptosis in vitro.Conclusion: Our data showed that cardiac circRNAs dysregulation is an important characteristic of viral myocarditis. |
| first_indexed | 2024-12-12T23:31:00Z |
| format | Article |
| id | doaj.art-ada20b17accb43f19a97419584618a1e |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-12T23:31:00Z |
| publishDate | 2022-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-ada20b17accb43f19a97419584618a1e2022-12-22T00:07:48ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2022-02-011010.3389/fcell.2022.760509760509Identification of Cardiac CircRNAs in Mice With CVB3-Induced MyocarditisXiang NieJiahui FanHuihui LiJin WangRong XieChen ChenDao Wen WangBackground: Viral myocarditis could initiate various immune response to the myocardium, resulting in myocyte damage and subsequent cardiac dysfunction. The expression profile and functions of circRNAs in this process are unknown.Methods: Fulminant myocarditis (FM) and non-FM models were induced by coxsackie B3 virus (CVB3) infection in A/J mice and C57BL/6 mice, respectively. CircRNAs expression profile was identified by RNA-seq. Quantitative RT-PCR, Spearman rank correlation, KEGG pathway, GO analysis, Western blot and flow cytometry were performed for functional analysis.Results: Severer inflammatory cell infiltration and cardiomyocyte necrosis were presented in CVB3-treated A/J mice than those in C57BL/6 mice. The dysregulated circRNAs in both of the mouse strains displayed strong correlation with the immune response, but dysregulated circRNAs in A/J mice were more prone to cardiac dysfunction. KEGG analysis indicated that the target genes of dysregulated circRNAs in A/J mice were mainly involved in viral infection, T cell and B cell receptor signaling pathways, while the target genes of dysregulated circRNAs in C57BL/6 mice were unrelated to immune pathways. Furthermore, knockdown of circArhgap32 that was downregulated in CVB3-treated A/J mice promoted cardiomyocyte apoptosis in vitro.Conclusion: Our data showed that cardiac circRNAs dysregulation is an important characteristic of viral myocarditis.https://www.frontiersin.org/articles/10.3389/fcell.2022.760509/fullfulminant myocarditisCircRNAscoxsackie B3 virusinflammationacute myocarditis |
| spellingShingle | Xiang Nie Jiahui Fan Huihui Li Jin Wang Rong Xie Chen Chen Dao Wen Wang Identification of Cardiac CircRNAs in Mice With CVB3-Induced Myocarditis Frontiers in Cell and Developmental Biology fulminant myocarditis CircRNAs coxsackie B3 virus inflammation acute myocarditis |
| title | Identification of Cardiac CircRNAs in Mice With CVB3-Induced Myocarditis |
| title_full | Identification of Cardiac CircRNAs in Mice With CVB3-Induced Myocarditis |
| title_fullStr | Identification of Cardiac CircRNAs in Mice With CVB3-Induced Myocarditis |
| title_full_unstemmed | Identification of Cardiac CircRNAs in Mice With CVB3-Induced Myocarditis |
| title_short | Identification of Cardiac CircRNAs in Mice With CVB3-Induced Myocarditis |
| title_sort | identification of cardiac circrnas in mice with cvb3 induced myocarditis |
| topic | fulminant myocarditis CircRNAs coxsackie B3 virus inflammation acute myocarditis |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2022.760509/full |
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